【正文】 ct ........................................................................................................................ II 第 1 章 緒論 ................................................................................................................ 1 課題背景 ........................................................................................................... 1 壓電傳感器的發(fā)展史 ....................................................................................... 2 壓電生物傳感器的研究進(jìn)展 ........................................................................... 3 論文研究?jī)?nèi)容 ................................................................................................... 4 第 2 章 壓電生物傳感器與凝血因子檢測(cè)系統(tǒng) ........................................................ 6 硅材料 ............................................................................................................... 6 壓電石英晶體傳感器的基本原理 ................................................................... 6 壓電石英晶體 ............................................................................................ 6 切型及金屬電極的選擇 ............................................................................ 8 Sauerbery 方程 ............................................................................................ 8 振蕩電路 .................................................................................................... 9 液相壓電傳感理論 .................................................................................... 9 凝血因子檢測(cè)系統(tǒng) ......................................................................................... 12 凝血因子檢測(cè)方法 .................................................................................. 12 石英振子的加工制備 .............................................................................. 13 壓電傳感器檢測(cè)池的設(shè)計(jì) ...................................................................... 14 檢測(cè)儀的設(shè)計(jì) .......................................................................................... 15 本章小結(jié) ......................................................................................................... 16 第 3 章 壓電石英傳感器頻率測(cè)量技術(shù)的實(shí)現(xiàn) ...................................................... 17 頻率的測(cè)量 ..................................................................................................... 17 等精度頻率的測(cè)量技術(shù) .......................................................................... 17 等精度頻率測(cè)量的基本原理 .................................................................. 17 等精度測(cè)頻的測(cè)量誤差 .......................................................................... 19 石英 晶體傳感器凝血因子 頻率檢測(cè) .......................................................... 19 壓電石英晶體傳感器等精度頻率測(cè)量的硬件實(shí)現(xiàn) .............................. 19 壓電石英晶體傳感器等精度測(cè)頻的軟件實(shí)現(xiàn) ....................................... 25 本章小結(jié) ......................................................................................................... 26 第 4 章 壓電傳感器頻率動(dòng)態(tài)響應(yīng)模型的建立 ...................................................... 27 凝血反應(yīng)體系粘度密度變化規(guī)律 ................................................................. 27 壓電石英晶體傳感器頻率變化與性質(zhì)的關(guān)系 ............................................. 28 粘度密度乘積變化與△ F 的關(guān)系 .................................................................. 28 粘度密度乘積變化與△ F 的關(guān)系 .................................................................. 28 本章小結(jié) ......................................................................................................... 30 xx 大學(xué)學(xué)士學(xué)位論文 IV 結(jié)論 ............................................................................................................................ 31 致謝 ............................................................................................................................ 32 參考文獻(xiàn) .................................................................................................................... 33 附錄 A ........................................................................................................................ 33 附錄 B ........................................................................................................................ 40 xx 大學(xué)學(xué)士學(xué)位論文 1 第 1章 緒 論 課題背景 現(xiàn)代生物分析技術(shù)研究和發(fā)展的一個(gè)重要目標(biāo)就是努力簡(jiǎn)化分析方法，使之能夠在普通和常規(guī)條件下進(jìn)行，并盡量減少操作技巧。 關(guān)鍵詞 壓電石英晶體；生物傳感器； 檢測(cè) xx 大學(xué)學(xué)士學(xué)位論文 II Studies of Biosensor Based on Piezoelectric Quartz Crystal Abstract The analyses of coagulation factor has been widely used in the clinical examination, now all kinds of detection methods based on agglutination, the end of the blood agglutination is judged by the viscosity and density of reaction system during blood agglutination. The end of the blood agglutination is judged by automated equipment or visual observation. The error of detection is big and poor repeatability by visual inspection and stopwatch time. The results obtained by automation equipment is accurate, rapid and reproducible, but the equipment is expensive and testing cost is high, so a sensitive, simple, precise measurement, lowcost new detection method is developed. Piezoelectric quartz resonator measurement technique can accurately measure the small change in the microcosmic reaction process and can be transformed into quantitative detection frequency signal, with subng level quality inspection capabilities, so a simple and fast method is provided for obtaining the abundant online realtime information of response system. Combined with the blood agglutination, through appropriate biological processing and structural design, we propose a plasma coagulation factor detection system sensor based on piezoelectric quartz crystal technology. The frequency count of hardware structure of piezoelectric crystal biosensor is designed based on microcontroller, using equal precision frequency measure. Using piezoelectric quartz crystal sensor oscillation frequency detection system for the platform, we establish piezoelectric sensor frequency dynamic re