【正文】
PNA ISH Detection ( 主要有檢測 EBV, Kappa, Lambda, Telomere的熒光標(biāo)記探針) nDNA Probes and DNA ISH Detection ( 主要為檢測 HPV) Molecular PathologynB and T Cell Gene Rearrangement Probes for Souther Blotting and Chemiluminescent System for Nucleic acid Blotting原理:用熒光標(biāo)記探針做 Southern或 Northern雜交,再用標(biāo)記 AP的抗熒光的抗體去檢測,由于 AP能水解底物 dioxtanephosphate,在探針處發(fā)出化學(xué)光并被膠片所捕捉,通過 CCD照相系統(tǒng)顯影特點(diǎn) : 無放射性 較 P32方法暴光時間短 使用標(biāo)準(zhǔn)膠片ISH Catalyzed Signal Amplification(CSA) SystemDAKO GenePointTM for Biotinylated Nucleic Acid Probes原理 :Step1: Step2: Step3: Step4: Step5: biotinylated peroxidase biotinyl deposition applicationprobe conjugated tyramide of biotinyl of DA