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(above ug/g) PNMElastase % % (above ) Calprotectin % % (above 6 ug/g) Lysozyme % % (above ug/g) IBDScan: A mercial quantitative ELISA for measuring fecal lactoferrin. There is a need for a rapid test for lactoferrin. ? An immunochromatographic test using polyclonal antibodies: a sandwich immunoassay with colloidal gold as visualization reagent. Instruments which allow for quantitation Instruments which allow for quantitation Flow through membrane test for detecting S. japonicum antibodies T Test spot Antigen Control spot Protein A C Test Procedure membrane serum Flow through membrane test for detecting S. japonicum antibodies T C * Specific antiS. japonicum antibodies are captured on the antigen test spot. * Serum antibodies are nonspecifically captured on Protein A control spot via its Fc Y Y Fc Flow through membrane test for detecting S. japonicum antibodies T C 3. Wash 4. Add ProteinA Gold 5. Wash Y Y Fc A A Red spot on T indicates a positive result. Absence of red spot indicates negative. Red spot on C indicates correct procedure. Absence indicates wrong procedure. Y Y =Serum antibody(nonspecific) =Specific antibody Procedure continued… Thank you The staff of MedTrend Diagnostic Forum from