【正文】 refold the rebinant protein . ?4. 歲月是無情的，假如你丟給它的是一片空白，它還給你的也是一片空白。 We will predict human Sun 139。 Accumulated rich experiece, I really want to have my own enterprise in order to prove my ability, which are also my parents’ willing. In September 2002, I was enrolled to study in ****** School in Beijing, with major of Business Administration (in English) But I found myself lack of advanced knowledge business. To study human Sun 139。歲月是有情的，假如你奉獻(xiàn)給她的是一些色彩，它奉獻(xiàn)給你的也是一些色彩。 With the aid of NMR and Xray crystallography, we can study directly proteindrug interactions and proteinprotein interactions. ? So when I graduated from senior high school, I chose to pursue professional knowledge about business. So to study abroad emerges in my minds. s 3D structure and molecular interactions by NMR or Xray crystallography. OVERVIEW OF THE STUDY Human Sun 139。你必須努力，當(dāng)有一天驀然回首時(shí)，你的回憶里才會(huì)多一些色彩斑斕，少一些蒼白無力。 NP_079430 ). Sun1 polyclonal antibodies will be produced against this peptide in rabbit. The antiSun1 serum IgG fraction (referred as the Sun1 serum) will be purified on a protein ASepharose column. 2. Rebinant Proteins Sun1 was initially isolated from human brain. SUN will be PCRamplified from the Sun1 cDNA and directly cloned in TOPO/V5His and pET21 vectors. Rebinant pET vectors will be transformed into BL21(DE3)plysS host which is ideally stringent to express membrane proteins. Verify if rebinant protein is expressed in inclusion bodies and purify it by Ni column. 3. Gel electrophoresis and Immunoblotting Test the dilution first and then use the Sun1 serum as the primary antibody. 4. Proteinase K Protection Assays The Sun1 cDNA