【正文】 7)顯色:滴加DAB反應(yīng)液,室溫下反應(yīng)5—10min。. 200х圖2多核巨細(xì)胞性肺炎 圖3 圖4 圖5 淋巴結(jié)炎 免疫組織化學(xué)染色 圖6 淋巴結(jié)炎 3 討論本次試驗(yàn)所選6只病犬年齡均小于3個(gè)月,屬于急性犬瘟熱感染病例。B細(xì)胞在淋巴小結(jié)和邊緣被膜下淋巴竇中數(shù)量最多。我要在這里對(duì)他們表示深深的謝意！ 感謝我的指導(dǎo)老師李國(guó)旺老師，沒有您的悉心指導(dǎo)就沒有這篇論文的順利完成。認(rèn)為犬瘟熱病毒包涵體并不都對(duì)犬瘟熱病毒的抗體免疫陽性反應(yīng),有可能是因?yàn)槿翢岵《景w中包括的是大量的病毒元件,這有可能會(huì)阻礙了特殊的單克隆抗體和病毒的抗原的結(jié)合。（見圖3）膀胱：膀胱粘膜完整,在400倍光學(xué)顯微鏡下可以觀察到,在犬膀胱粘膜變移上皮中出現(xiàn)犬瘟熱病毒包涵體。3)封閉內(nèi)源性酶:在組織切片上,滴加3%過氧化氫,以組織完全覆蓋為宜,在室溫下處理20min。s distemper antigen is strong positive, and higher density. And in the lungs although dog show serious pneumonia symptoms, but canine distemper virus antigen positive reaction is weak, only in the bronchial epithelium and a small amount of macrophages observed in the cytoplasm. Canine distemper virus first in dogs in the lymphatic organization reproduced proliferation, damaging the subcapsular sinus and lymphatic section of T lymphocytes and B lymphocytes, influence lymphoid tissue output to peripheral blood circulating lymphocyte quantity. Conclusion: canine distemper virus disease to clinical lung lesions though most serious, but acute type and subacute type of canine distemper virus mainly in lymphoid tissue T lymphocytes B lymphocytes and macrophage proliferation, so should be based in lymphoid tissue as a canine distemper virus39。s distemper acute cases lymph nodes cortex your dog39。2)抗原修復(fù):,。高倍鏡下,觀察到皮質(zhì)區(qū)被膜下淋巴竇和皮質(zhì)淋巴竇中,有大量吞噬異物的巨噬細(xì)胞,網(wǎng)狀內(nèi)皮細(xì)胞增生,