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Linearity and precision were tested using the following kits:UVTrol (Biomerieux France) and Holnicob (Biotrol France). Method All analyses were performed at either 30C or 37C. The instrument settings were those remended by the oxaloacetic transaminase (SGOT) and pyruvic transaminase (SGPT) were measured by the Wroblewski method at 37C or by the remended method of the SFBC at 30C with the Biomerieux kits. Alkaline phosphatase (ALP) was measured following the remended procedure of the German Society of Clinical Biochemstry (DGKC) at 37C. Accuracy, within day and day to day, and carryover were tested using low, medium and high concentration control sera spiked where necessary with animal enzymes. These control sera were furnished by a local association operating in biological measurement quality control. (Pro Bio. Qual, Lyon, France). Results and discussion Accuracy and precision of the Olli D sample processor The results are presented in Table 1. Precision expressed as the coefficient of variation for 24 determinations was never greater than % for the most important dilution (10 of drawing ink in 510 1 of water). The measured absorbances varied directly with the amount of drawing ink added and were indistinguishable from those obtained by manual dilution. Daytoday precision was % for the 1/12 dilution used routinely for SGOT and SGPT and % for the 1/30 dilution used routinely for ALP. 咸寧學(xué)院本科畢業(yè)設(shè)計(jì)(論文):全自動(dòng)生化分析儀的維護(hù)與保養(yǎng) 3 The absorbance of drawing ink is temperature maximum variation for a temperature range of 2040C and an absorbance of was + . Temperature control With the different automatic analysers the solution of paranitrophenol gave an average inverse ratio of absorbance units for an increase of 1C. With the Olli C, the absorbances found were + at 25C and + at 37C, or absorbance units/degree. The desired termperature was reached rather slowly. If the blocks and cuvettes were preheated to the designated temperature of 37 C, the usual volume of paranItrophenol O (at the lmtlal temperature of + 4 C) reached 37 C in 8 minutes in the Olli C and 7 minutes in the incubator (Figure 1).Similar results (not shown) were found for a set temperature of 30C, the steady state was reached in 6 minutes for the Olli C, and 5 minutes for the incubator. The block has to be manipulated outside the apparatus,and it is therefore impor_tant to know the rate of cooling. At a set temperature of 37C, the block outside the apparatus cooled by during the first minute and then C/minute during the following 5 minutes. Measurements were made with a temperature probe at ambient temperature. To avoid this cooling when the blocks were manipulated outside the incubator for either the Olli C or the Olli D, the set temperature in the incubator was set slightly higher, for 30C and for 37C. Accuracy and precision of the photometer Linear absorption was maintained up to units of absorbance at 340 nm and up to at 405 nm. Accuracy and precision, tested at 340 nm with the UV Trol kit (stabilized solution of NADH) were both good. Similar results at 660 nm were found with the Holnicobkit (cobalt nitrate). Analytical results precision Withinday and daytoday precision measurements, precision for SGOT measurements at 37C are presented in Tables 2 and 3. The CV did not exceed % at any of the levels of serum activity studied. The SGPT and ALP values,measured at 37C, (valued not shown) were similar. SFBC remendations specify withinday precision for SGOT to be within % at the low le