生物醫(yī)學(xué)工程外文翻譯-生物工程-wenkub.com
2025-01-15 03:25 本頁面
【正文】 中等水平 ?？?速 決定和 降 低分析估計(jì)成本 . 例如 Olli C+D 平行分析者 ((Kone Oy 工具部門 Espoo,芬蘭 )回答這些標(biāo)準(zhǔn) .一 些 關(guān)于 Olli C 系統(tǒng)的報(bào)告已經(jīng)已經(jīng)被被 Puuka 出版和分量足的 [1].超過一年這評價(jià)處理它的仔細(xì)和每天使用在朝派酶學(xué)剩余物一時(shí)期耐久使用 C+Dsystem. 緒論： 工具是一個(gè)平行 ,多信道的 ,光度學(xué)的分析者 .決定 反應(yīng) 的不同階段履行在朝派批包含空白 ,標(biāo)準(zhǔn)和控制手段 24 樣品 .21A 分鐘分析的速度是根據(jù)根據(jù)小時(shí)反應(yīng)時(shí)間存在 360 運(yùn) 動(dòng)的度量的小時(shí)有的端點(diǎn)決定 700 個(gè)試驗(yàn) . 分析者包括三個(gè)獨(dú)立單位 ,樣品稀釋者 //處理器 ,(每個(gè)被微處理機(jī) )和孵化器控制光度計(jì) .樣品改變被手履行使用在一 thermostatedblock 中可處理的透明小容器 . Olli D 稀釋者尺寸為 350 x1100 x740 毫米和重量是 70 千克 .它存在編造的a,用 24 透明小容器起跑器 ,其安排 Olli C 光度計(jì) ,八艘試劑船 ,有 24 漿液小瓶的一起跑器 ,一配發(fā)頭兒和一根手指八小費(fèi)的配發(fā)盤子作炫耀行為和鍵盤 .稀釋者能被手為編制程序或者在附近磁帶盒為 30 細(xì)察 。 Miniken (Coultronic),Cobas BIO (Roche,) PA 800 (Vitratron), ACP 5040 (Eppendorf) On the Olli C, the time needed to reach the set temperature of 30C or 37C with an analysis volume of 550 ul(contained in the acrylic cuvette in the thermostated block)was followed by noting the decrease in the absorbance of the paranitrophenol solution. The temperatures of cuvette contents were also monitored with a Metrix temperature probe (HA 1159). Optical performance This was tested according to the procedure of Rand [5].Linearity and precision were tested using the following kits:UVTrol (Biomerieux France) and Holnicob (Biotrol France). Method All analyses were performed at either 30C or 37C. The instrument settings were those remended by the oxaloacetic transaminase (SGOT) and pyruvic transaminase (SGPT) were measured by the Wroblewski method at 37C or by the remended method of the SFBC at 30C with the Biomerieux kits. Alkaline phosphatase (ALP) was measured following the remended procedure of the German Society of Clinical Biochemstry (DGKC) at 37C. Accuracy, within day and day to day, and carryover were tested using low, medium and high concentration control sera spiked where necessary with animal enzymes. These control sera were furnished by a local association operating in biological measurement quality control. (Pro Bio. Qual, Lyon, France). Results and discussion Accuracy and precision of the Olli D sample processor The results are presented in Table 1. Precision expressed as the coefficient of variation for 24 determinations was never greater than % for the most important dilution (10 of drawing ink in 510 1 of water). The measured absorbances varied directly with the amount of drawing ink added and were indistinguishable from those obtained by manual dilution. Daytoday precision was % for the 1/12 dilution used routinely for SGOT and SGPT and % for the 1/30 dilution used routinely for ALP. 咸寧學(xué)院本科畢業(yè)設(shè)計(jì)（論文）：全自動(dòng)生化分析儀的維護(hù)與保養(yǎng) 3 The absorbance of drawing ink is temperature maximum variation for a temperature range of 2040C and an absorbance of was + . Temperature control With the different automatic analysers the solution of paranitrophenol gave an average inverse ratio of absorbance units for an increase of 1C. With the Olli C, the absorbances found were + at 25C and + at 37C, or absorbance units/degree. The desired termperature was reached rather slowly. If the blocks and cuvettes were preheated to the designated temperature of 37 C, the usual volume of paranItrophenol O (at the lmtlal temperature of + 4 C) reached 37 C in 8 minutes in the Olli C and 7 minutes in the incubator (Figure 1).Similar results (not shown) were found for a set temperature of 30C, the steady state was reached in 6 minutes for the Olli C, and 5 minutes for the incubator. The block has to be manipulated outside the apparatus,and it is therefore impor_tant to know the rate of cooling. At a set temperature of 37C, the block outside t
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