【正文】 s leadership and the party39。s leadership, to solve the masses of the people reflect a focus on the problem. The new revision of the code and rule , reflects the party39。2)然后將瓶口密封，進(jìn)入發(fā)酵。7. 貯藏：滿瓶、調(diào)游離S02為20～30mg/L。：用斐林試劑法測(cè)定還原糖，用堿滴定法測(cè)定總酸。將發(fā)酵液微熱到40℃左右，降低發(fā)酵液的黏度，將其用紗布過(guò)濾，得濾液。利用這一特點(diǎn)，；發(fā)酵產(chǎn)物中檸檬酸為多鹽有機(jī)酸，能與CaCO3形成沉淀，利用鈣鹽法即可檢測(cè)。：先用低倍鏡觀察菌絲有無(wú)隔膜，孢囊梗的形態(tài)，孢囊的著生方式，孢囊和囊軸的形態(tài)和大小。隨著發(fā)酵時(shí)間延長(zhǎng)，甜酒釀中的糖分逐漸轉(zhuǎn)化成酒精，因而糖度下降．酒度提高，故適時(shí)結(jié)束發(fā)酵是保持甜酒釀口味的關(guān)鍵。⑶分離純化：乳酸菌的分離可采用新鮮酸奶進(jìn)行平板涂布分離，或直接用接種環(huán)蘸取酸奶作劃線分離?；蛴檬惺埘r牛奶加5%蔗糖調(diào)勻也可。實(shí)驗(yàn)一 培養(yǎng)基的配制及滅菌一、實(shí)驗(yàn)?zāi)康囊笳莆詹煌愋臀⑸锱囵B(yǎng)基的配方及其制備方法。若經(jīng)提問(wèn)發(fā)現(xiàn)沒(méi)有預(yù)習(xí)者，須在教師指定的時(shí)間內(nèi)預(yù)習(xí)完畢，方得參加實(shí)驗(yàn)。如有破損須報(bào)告指導(dǎo)教師，并填寫(xiě)儀器損壞登記簿。由于酸奶中含有乳酸菌的菌體及代謝產(chǎn)物，因而對(duì)腸道內(nèi)的致病菌有一定的抑制作用；對(duì)人體的腸胃消化道疾病也有良好的治療效果。⑼貯存 產(chǎn)品的貯存溫度為2℃~6℃。進(jìn)一步掌握微生物的分離、培養(yǎng)等基本方法和無(wú)菌操作技術(shù)。3. 觀察平板上的菌落形態(tài)，用接種環(huán)調(diào)取霉菌菌落的孢子或菌絲體于新鮮的馬鈴薯蔗糖瓊脂平板上，再進(jìn)行劃線培養(yǎng)，直至獲得純培養(yǎng)，即平板上只有一種霉菌的菌落或菌苔。適合于固體發(fā)酵法生產(chǎn)檸檬酸的原料諸如：甘薯渣、木薯渣、蘋(píng)果渣和甘蔗渣等。待酵母活化后，按10%將其接種到裝有原料的三角瓶中。計(jì)算：在發(fā)酵結(jié)束后還需要再進(jìn)行出汁率的測(cè)定。：每天測(cè)兩次比重、溫度，并定期用木柄壓“帽”、用冷水噴淋或在空調(diào)室內(nèi)控溫至26～30℃。 將晾干的葡萄用粉碎機(jī)粉碎（用手捏碎）。 the third part introduces two party regulations modified the main changes and needs to grasp several key problems。s selfdiscipline norms and party members and leading cadre clean fingered selfdiscipline norms, a total of eight, collectively referred to as the eight. Four must and eight of the content from the party constitution and Party39。 at the same time, to investigate violations of Party members and even criminal behavior of Party discipline and responsibility, Regulations distinguish five different conditions, with special provisions were made provisions, so as to realize the connection of Party discipline and state law. 4, reflect Wind building and anticorruption struggle of the latest achievements. rule the party39。 the second is concisely positive advocated by the lack of prohibited provisions excessive, no autonomy requirements。先將葡萄洗干凈，晾干水分，用手捏碎放進(jìn)廣口瓶?jī)?nèi)，每放一部分葡萄放一層白糖，葡萄只能裝到容器的2/3處，自然氣溫在2030℃為最佳。7d后當(dāng)酒液糖度5176。10. 裝瓶：℃左右，在同溫條件下進(jìn)行澄清、除菌過(guò)濾。除梗，破碎30%，入罐。實(shí)驗(yàn)六、釀酒葡萄成熟度的控制以及入罐發(fā)酵一、目的與要求 成熟度是決定葡萄酒質(zhì)量的重要因素。接種：將培養(yǎng)基趁熱打散，待降溫到37℃，即可將黑曲霉孢子接種到其中，振蕩混勻。五、實(shí)驗(yàn)報(bào)告 實(shí)驗(yàn)結(jié)果(1)發(fā)酵期間每天觀察、記錄發(fā)酵現(xiàn)象。我國(guó)釀酒工業(yè)中的小曲酒和黃酒生產(chǎn)中的淋飯酒在某種程度上就是由甜酒釀發(fā)展而來(lái)的。 表1項(xiàng)酸奶中的各種乳酸菌在馬鈴薯汁牛奶培養(yǎng)基平板表面通常呈現(xiàn)三種形態(tài)特征的菌落：①扁平型菌落：大小為23mm，邊緣不整齊，很薄，近似透明狀，染色鏡檢為細(xì)桿菌；②半球狀隆起菌落：大小為12mm，隆起成半球狀，邊緣整齊且四周可見(jiàn)酪蛋白水解透明圈，染色鏡檢為鏈球狀；③禮帽形突起菌落：大小為12mm，邊緣基本整齊，菌落中央呈隆起狀，四周較薄，有酪蛋白透明圈，染色鏡檢呈鏈球狀。⑷冷卻 將已滅菌的牛奶冷卻到4045℃。 霉菌常用培養(yǎng)基PDA（馬鈴薯蔗糖培養(yǎng)基） 去皮馬鈴薯200g切成小塊，加水1000ml煮沸30min出汁，三層紗布過(guò)濾，濾液中加入2%蔗糖，補(bǔ)充水至終體積1000ml，加瓊脂2%，自然pH。對(duì)玻璃器皿須做好清洗工作。不得抄寫(xiě)他人的實(shí)驗(yàn)實(shí)習(xí)記錄，否則，須重做。二、基本原理酸奶是以牛乳為主要原料，接入一定量乳酸菌，經(jīng)發(fā)酵后制成的一種具有較高營(yíng)養(yǎng)價(jià)值和特殊風(fēng)味的發(fā)酵乳制品飲料。感官檢驗(yàn)試驗(yàn)方法① 色澤和組織狀態(tài)：取適量試樣于50mL燒杯中，在自然光下觀察色澤和組織狀態(tài)。嗜熱鏈球菌生長(zhǎng)過(guò)程中，乳脲活動(dòng)產(chǎn)生CO甲酸刺激保加利亞乳桿菌生長(zhǎng)。 應(yīng)符合表2的規(guī)定。 ≤大腸菌群，MPN/100Ml批次品 評(píng) 項(xiàng) 目凝乳情況乳清淅出狀態(tài)稀薄粘度表面氣泡沫表面光澤口感酸度香味異味發(fā)粘pH結(jié)論1234將單菌和混菌發(fā)酵的酸奶品評(píng)結(jié)果記錄于下表中。釀成的甜酒釀應(yīng)是醪液清澈半透明而甜醇爽口。前兩種方法利用空氣氣相中的氧氣，后者則是利用液體中的溶解氧。檸檬酸固體發(fā)酵過(guò)程中應(yīng)注意哪些操作要點(diǎn)？實(shí)驗(yàn)五 酒精發(fā)酵及下游處理實(shí)驗(yàn)一、實(shí)驗(yàn)?zāi)康恼莆站凭l(fā)酵工藝的具體操作。簡(jiǎn)單的成熟度的測(cè)定可用手持糖量計(jì)測(cè)定，如果是精確的測(cè)定可在實(shí)驗(yàn)室中采用斐林試劑測(cè)定。2. 裝瓶：裝量不超過(guò)瓶容的75％，同時(shí)按汁量加入50～80mg/LS02攪勻，亞硫酸的濃度為6%，6%，添加量的計(jì)算（）：[葡萄果實(shí)kg50]/[1000]ml。四、討論1. 在酒精度的測(cè)定中需要進(jìn)行校正。 注意： 成品葡萄酒指標(biāo)：糖酸酒精顏色單寧（g/L）pH（1112）第 62 頁(yè) 共 62 頁(yè)（英文版 ） Two regulations promulgated for implementation is in the party in power for a long time and the rule of law conditions, the implementation of prehensive strictly strategic plan, implementation in accordance with the rules and discipline to manage the party, strengthen innerparty supervision of major initiatives. The two regulations supporting each other, the code adhere to a positive advocate, focusing on morality is of Party members and Party leading cadres can see, enough to get a high standard。s eighteen years strict political discipline and political rules, organization and discipline and to implement the central eight provisions of the spirit against the four winds and other requirements into Disciplinary provisions. Not one pace reachs the designated position, focusing on in line with reality, pragmatic and effective. After the revision of major changes, major changes in the code and rule modified and needs to grasp several key problems (a) code code adhere to according to regulations governing the party and party with morals in bination, for at the present stage, the leadership of the party members and cadres and Party members in existing main problems of selfdiscipline, put forward principles, requirements and specifications, showing Communists noble moral pursuit, reflected at all times and in all over the world ethics from high from low 5 mon requirements. One is closely linked to the selfdiscipline, removal and no direct relation to the provisions of . the second is adhere to a positive advocate, eight prohibition 52 are not allowed to about the content of the negative list moved into synchronization amendment cases . Three is for all the party members, will apply object from the leadership of the party members and cadres to expand to all Party members, fully embodies the prehensive strictly required. The fourth is prominent key minority, seize the leadership of the party members and cadres is the key, and put forward higher requirements than the ordinary Party members. Five is to simplify, and strive to achieve concise, easy to understand, easy to remember. The revised code is the ruling Party since the first insists on a positive advocate forAll Party members and the selfdiscipline norms, moral declaration issued to all members of the party and the National People39。s purpose. 8 of these regulations, a total of three series, Chapter 15, 178, more than 24000 words, after the revision of the regulations a total of 3 series, Chapter 11, 133, 17000 words, divided into general and special provisions and Supplementary Provisions Part 3. Among them, add, delete, modify the provisions of the proportion of up to nearly