hbvdna定量檢測(更新版)
【正文】 準(zhǔn)確、客觀地闡述了 PCR方法。 The software displays the fluorescence signals in realtime immediately after each measurement. Signals from the samples are obtained as the machine positions the capillaries sequentially over the optical unit. During thermal cycling, fluorescence can be measured once per cycle for every capillary and the fluorescence values are displayed immediately on the screen and updated after every cycle (). The generated data are stored for further analysis. 熒光定量 PCR在每一次循環(huán)后對進(jìn)行實時監(jiān)控，因此能最準(zhǔn)確反映出對數(shù)期。 Ct值 ， C代表 Cycle， t代表 threshold， Ct值的含義是：每個反應(yīng)管內(nèi)的熒光信號到達(dá)設(shè)定的域值時所經(jīng)歷的循環(huán)數(shù) 。s flexible programming allows the user to acquire fluorescence above the melting temperature of the primer dimers, but below the melting temperature of the product. 缺點：存在非特異性產(chǎn)物，可以與非特異性產(chǎn)物，甚至引物二聚體結(jié)合發(fā)出熒光
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