【正文】
tion is a hotspot of research and development at home and abroad. The study found that cadmium absorbed by the body will turn into cadmium sulfide protein, cadmium sulfide is a selective protein, can be accumulated in kidney, a third of the cadmium can be absorbed by the kidney, the kidney is the target an of cadmium poisoning. Other ans such as pancreas, spleen, thyroid gland and hair also be accumulated a certain amount. Cadmium in the body will be united by protein molecules containing amino, hydroxyl, inhibition of many enzyme system, so that the normal function of the liver and kidney ans in enzyme system will be damaged. Due to the cadmium, the patient can appear the symptom of diabetes, protein and amino acid urine, More seriously, which can make bone metabolism, cause osteoporosis, contraction and a series of symptoms, the harm of cadmium has been greatly concerned. In this study, Chelating peptide content in plants was used as an index, which explored the influence that different concentrations of cadmium induced the content of peanut plant chelating peptide, the study use peanuts grown in different heavy metal chromium as raw material and take advantage of high performance liquid chromatographymass spectrometry instrument to determine the content of peanut plant in chelating peptide. This experiment took advantage of reversed phase column C, acetonitrile and % trifluoroacetic acid, which detected the chelating peptide content of peanut under The induced of different concentrations of cadmium. In the above experimental conditions, the detection limit was mg/L。其機理是:重金屬離子植物 吸收到體內后,與細胞內的 PC 作用構成復合物 , 而后轉運到特定的細胞器 ( 特別是液泡 ),然后區(qū)室化絡合 , 降低細胞質中有毒的金屬離子的濃度。曾有學者用實驗測定了金屬離子對 PC 的合成的誘導能力比較 , 結果顯示其中 Cd2+對 PC 合成的影響最大,同時 Ni2+、 Cd2+、 Cu2+、 Hg2+、Ag+、 Sn2+、 Zn2+、 Sb3+、 P2+、 Au+均可誘導 PC 合成,然而將激活劑金屬離子濃度降低或者是外加金屬螯合劑 ( 例如 EDTA)時, PC 的合成就會被迫停止,這說明 PC 生物合成需要金屬離子的誘導,同時實驗表明不同金屬對 PC 誘導作用在不同種植物中有 不同的強度。植物對 Cd2+解毒方法可以分為硫含量低的 PcCd 復合物的跨液泡膜轉運、硫離子在液泡中的累積導致的含硫量高的 pCCdS 形成這兩種方式。 花生中 GSH, PC 等總含量的測定 配置含 mmol /L 的埃爾曼試劑試劑顯色劑 (含 mmol /L 的 DTPA)的 200 mmol /L 的 HEPPS 緩沖液( pH = ),避光保存 , 取花生組織提取液 300 L 加到 300L DTNB 顯色劑中,混勻后在 30℃下反應 3 min,其中 GSH, PC 和 TNB 會發(fā)生反應,生成黃色化合物,非蛋白巰基化 合物與黃色化合物含量符合 1:1 的 比例關系, 依據(jù)有差別的花生樣品在 420 nm下的吸光度值不同,便可計算出花生樣品中巰基含量。優(yōu)化正離子 9 掃描條件下 各種條件,例如錐電壓為 : 25 V; 噴霧電壓為 : kV; 溶劑氣體溫度的去除: 350℃ ; 采樣錐電壓 : 20 V; 透鏡電壓 V; 錐孔溫度為 : 105℃ ; 脫溶劑氣流量為 : 110m L/h ; 全掃描范圍 m /z 為 : 5001200,反吹氣流量為 : 80m L /h。 表格 實驗的回收率與精密度的分析結果 ( n = 3) NUMBER 分析結果 mg/kg 回收率 (%) RSD 1 2 3 實際花生樣品的分析 選用 HPLCMS 檢測方法,在兩種不同鎘水平下測定了兩個花生