高分子材料研究方法凝膠色譜法(完整版)
【正文】 ile phase pump autoinjector column(s) detector(s) data acquisition Temperature control 柱溫控制 ? environment temperature less than 50 ?C ? highest control temperature less than 150 ?C ? different solvents, different temperature（ THF:35 ?C , DMF:85 ?C ） Separation Mechanisms ? The Fundamental Equation: ① C: the elution volume ② VM: the void volume of the mobile phase ③ VS: the calculative internal volume within the porous particles ④ K: the distribution coefficient ? 0 ≤ K ≤1 ? Very large molecules: K=0, VR = VM ? Small molecules: K=1, VR = VM + VS Separation Mechanisms ? Equilibrium exclusion theory: suitable for most situations Limited diffusion theory: high flow velocity Flow separation theory: very high flow velocity Data Treatment ? Gel Chromatograms ? Correction Curve of Molecular Weight ? Calculation of DMW ? Correction of Width of Peak Gel Chromatograms ? Plots: ? Horizontal axis: the retention values of the effluent ∝ logarithm of MW ? Vertical axis: concentration of eluent ? Monodispersive samples: Gaussian distribution curve ? Polydispersive samples: sum of distribution curves of monodispersive samples Correction Curve of Molecular Weight ? Keys: ? Transition from VR to MW
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鄂ICP備17016276號-1