【正文】 lopment of biological materials, especially in bone repair materials. Thanks to the bone repair material can directly or indirectly treat disease or auxiliary repair damaged bones, but also has excellent patibility and biodegradability of the anism, and excellent mechanical strength and antibacterial property. This scheme can optimize the treatment of bone disease, reduce the risk of treatment or operation, and after implantation with human metabolism and degradation, the operation time necessary to remove two. The bone repair material prepared by electrospinning has high specific surface area and porosity, which is very beneficial to human bone cell growth, adhesion, differentiation, promote bone repair. But there is a certain human bone structure, so the use of micro pattern template of electrospun fibers, the posite fiber has a certain structure, more conducive to play the function of . Therefore, the preparation of GE/CS/HA/GO four posite fiber scaffolds for bone repair materials by electrospinning technique and patterned templates. And to explore the four element posite fiber GE/CS/HA/GO electrospinning conditions and the best parameters. The characterization of its morphology and properties. The research content includes the following parts: (1) to explore the optimal concentration of GO, the preparation of four element posite fiber GE/CS/HA/GO under optimal conditions based on GE/CS/HA three system results show that: by light and electron microscopy were obtained when the GO concentration is % and the template for the 200 mesh, the surface of fiber arranged orderly, uniform thickness, no bead and drop. Better morphology. (2) when the materials implanted in the human body, in order to ensure the supply of nutrients required for cells to repair, bone defect, posite materials have a certain adsorption on protein. The adsorption amount of four element posite fiber will be prepared at the optimal conditions of the bovine serum albumin in human body fluid environment. The results show that: the saturated adsorption amount of four yuan of GE/CS/HA/GO posite fiber BSA in %. With the increase of BSA and the pH value of the solution, the adsorption order disordered fiber surface and microstructure of BSA fiber surface is similar, but the effective area of the protein adsorption is different, resulting in the maximum adsorption capacity of BSA on the surface of the material pH value is slightly different. The initial concentration of BSA, the initial adsorption amount of fiber is larger, but it is to achieve balance in 20 min. (3) posite fibrous scaffold after implantation in the human body, such as friction extrusion by the surrounding tissue, an. Therefore, the need to fine fibrous scaffolds with excellent mechanical properties and maximize its function. The four element posite fiber disordering and patterned GE/CS/HA/GO tensile strength test, and pared with the three element posite fiber GE/CS/HA. The results showed that: four yuan three yuan was significantly higher than that of posite fiber IV posite fiber, this is due to the addition of GO in the four element system, improve the quality of fiber bonding interface, which improves the tensile strength of. The four element posite fiber patterning of GE/CS/HA/GO tensile strength reached . Due to the anisotropic microstructure of posite fibers, the tensile strength of the tensile strength of the micro structure fiber membrane is greater than that of disordered fiber. Therefore, the posites exhibit good mechanical strength. The above research results indicate that, this paper successfully prepared GE/CS/HA/GO four element posite fiber, and to characterize the spatial distribution and arrangement, enhance the antibacterial properties and mechanical strength of the posite fiber. Is expected to make the bone repair materials play an important role in the field of clinical medicine. Keywords: graphene oxide。 生物材料的定義 生物 材料是一類用于人體骨骼、組織和器官的診斷、修復(fù)或增進(jìn)其功能的高科技材料，其功能直接作用于需治療部位，其作用藥物是不可替代的。因此國家實(shí)施了一系列政策和經(jīng)濟(jì)支持 ，刺激其產(chǎn)業(yè)化發(fā)展速度，增強(qiáng)我國生物材料的自主創(chuàng)新能力，減少進(jìn)口需求并加大出口力度，擴(kuò)大中國生物材料在國際上的影響力。主要有局部的組織反應(yīng)和全身的免疫反應(yīng)。因此，植入時應(yīng)嚴(yán)格消毒滅菌，避免植入失敗。 骨修復(fù)材料 將生物材料用于人體骨修復(fù)領(lǐng)域中，是近年來一個研究熱點(diǎn)。當(dāng) pH 等電點(diǎn)，明膠帶負(fù)電荷，向電場陽極移動，具有堿性。能發(fā)生水解、烷基化、?；?、羧甲基 化、磺化、硝化、鹵化、縮合和絡(luò)合等化學(xué)反應(yīng)，可生成許多具有不同性能的衍生物，擴(kuò)大了殼聚糖的應(yīng)用范圍。其結(jié)構(gòu)如圖 12 所示，它具有兩親性，從其邊緣到中央呈現(xiàn)親水至疏水的性質(zhì)分布 。因此，本實(shí)驗將采用靜電紡絲技術(shù)制備四元復(fù)合纖維。 在 GE/CS/HA 三元 體系 的最優(yōu)條件基礎(chǔ)上制備 GE/CS/HA/GO 四元復(fù)合纖維 ，利用光學(xué)顯微鏡和掃描電子顯微鏡對其形貌進(jìn)行研究，探索添加 GO 的最優(yōu)濃度， 以及圖案化模板的選 擇。將磨鈍的針頭固定在注射卡槽中，與注射器連接好，將電紡液注入干燥的注射器中，排空注射器中多余的空氣后注入導(dǎo)管。主要考察吸附時間、 BSA 溶液的 pH 和 BSA 的初始濃度對其影響。 ③ BSA 的初始濃度：用 pH為 的 PBS 溶液配制不同初始濃度的 BSA溶液。 將樣品裁剪成 1cm 1cm， 浸泡 在 10mL的 BSA 溶液 中。