中藥藥理研究思路與方法-文庫吧在線文庫
【正文】 expression was most sensitive to U0126.Figure3 Blocking ERK activation abolished the regulatory effect of BBR on LDLR.Figure3(b) BBR rapidly activated ERK and the kiics of ERK activation preceded the upregulation of LDLR expression by BBR.Figure3(c) The activation of ERK by BBR is also dose dependent.Figure4 BBR reduces plasma LDLc and increases liver LDLR expression in hamsters.Figure4(a) Figure4(b)Figure4(a):Treatment of hyperlipidemic animals with BBR resulted in dosedependent decreases in both serum total cholesterol and LDLc.Figure4(b): increase in mRNA and a increase in protein hamster livers treated with 100mg/kg/d of BBR.Figure5 BBR lowered serum LDLc in hypercholesterolemic peopleFigure6 BBR treatment also significantly improved liver function in this subgroup12Results conclusion:nOral administration of BBR in 32 hypercholesterilemic patients for 3 months reduced serum cholesterol by 29%,triglycerides by 35% and LDLcholesterol by 25%.nTreatment of hyperlipidemic hamsters with BBR reduced serum cholesterol by 40% and LDLcholesterol by 42%,with a increase in hepatic LDLR mRNA and a increase in hepatic LDLR protein.nUsing human hepatoma cells,BBR upregulates LDLR expression independent of sterol regulatory element binding proteins, but dependent on ERK activation.nBBR elevates LDLR expression through a posttranscriptional mechanism that stabilizes the mRNA.nUsing a heterologous system with luciferase as a reporter, the 5’ proximal section of the LDLR mRNA 3’ untranslated region responsible for the regulatory effect of BBR.nBBR as a new hypolipidemic drug with a mechanism of action different from that of statin drugs.v一個(gè)結(jié)合v二個(gè)基本講清v三個(gè)化學(xué)層次v四個(gè)藥理水平q中藥復(fù)方藥理研究總思路(一 )中藥復(fù)方組分配伍研究(二 )基于定向剔除技術(shù)的復(fù)方藥效物質(zhì)基礎(chǔ)研究(三 )中藥血清藥理學(xué)研究(四 )體外血腦屏障模型 研究......q中藥復(fù)方藥理研究方法 方劑是一個(gè)復(fù)雜體系，其作用的人體也是一個(gè)復(fù)雜系統(tǒng)，面對雙重復(fù)雜系統(tǒng)，必須在復(fù)雜性科學(xué)理論的指導(dǎo)下，將復(fù)雜系統(tǒng)中非線性規(guī)律部分降階、降維為線性規(guī)律去研究。 P vs model group** 30（ min）時(shí)間組分靶點(diǎn)作用15 30丹參酮類血管＋細(xì)胞抗氧化抗炎丹酚酸類血管擴(kuò)張冠脈改善血供三七總皂苷細(xì) 胞 保護(hù)心肌 改善功能中樞調(diào)節(jié)下丘腦龍 腦0 9060 15090炎性反應(yīng)相關(guān)基因信號轉(zhuǎn)導(dǎo)通路圖 全部基因的總圖免疫應(yīng)答相關(guān)基因信號轉(zhuǎn)導(dǎo)通路圖 32免疫應(yīng)答丹酚酸 B/三七總皂苷抗心肌梗死的分子機(jī)制細(xì)胞因子、信號轉(zhuǎn)導(dǎo)調(diào)控＋ Sal B/PNS配伍－177。但作為一種新興的實(shí)驗(yàn)方法
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