【正文】 of use prescribed in the discipline rectifying process, non convergence, not close hand classified as severely or heavier punishment. Discipline straighten At least eighteen years of five years, these five years is to pay close attention to the provisions of the central eight implementation and anti four winds . The fourth chapter on suspicion of illegal party disciplinary distinguish five different conditions, with special provisions were made。s close ties with the masses. Discipline to protect the party39。s basic theory, basic line, basic program and basic experience, the basic requirement of behavior made prescribed punishment, increase the cliques, against the organization such as violation of the provisions, to ensure that the central government decrees and the Party of centralized and unified. 3, adhere to strict discipline in the law and discipline In front, Ji separated. Revised Regulations adhere to the problem oriented, do Ji separated. Any national law existing content, will not repeat the provisions, the total removal of 79 and criminal law, repeat the content of the public security management punishment law, and other laws and regulations. In the general reiterated that party organizations and Party members must conscientiously accept the party39。s selfdiscipline norms and party members and leading cadre clean fingered selfdiscipline norms, a total of eight, collectively referred to as the eight. Four must and eight of the content from the party constitution and Party39。s ideals and beliefs, excellent traditional style. The revised the code closely linked to the selfdiscipline, insisting on the positive initiative, for all members, highlight the vital few, emphasized selfdiscipline, focusing on the morality, and the majority of Party members and the ideological and moral standards. The revised Ji method separately, Ji, Ji Yan to Method, as a negative list, emphasizing the heteronomy, focusing on vertical gauge. Is this one high and one low, a positive reaction, the strict party discipline and practice results transformation for the integration of the whole party to observe moral and discipline requirements, for the majority of Party members and cadres provides benchmarking and ruler. Third, insist on to. In view of the problems existing in the party at the present stage, the main problems of Party members and cadres in the aspect of selfdiscipline and abide by the discipline to make clearly defined, especially the party39。 the fourth is to clean the theme is not prominent, not for the existing problems, and is narrow, only needle of countylevel leading cadres above. rule is in 1997 Chinese Communist Party disciplinary cases (Trial) based on revision, in December 2003 the promulgation and implementation, to strengthen the construction of the party play very important role. Along with the development of the situation, which many provisions have been unable to fully meet the prehensive strictly administering the practice needs. One is Ji law, more than half of the provisions and criminal law and other countries laws and regulations Repetition。s 18 and the eighth session of the third, the spirit of the fourth plenary session, reflecting the experience of studying and implementing the General Secretary Xi Jinping series of important speech, reflects the party39。 the third part introduces two party regulations modified the main changes and needs to grasp several key problems。再用虹吸管將上面的酒液抽出，就成了晶瑩透亮的成品葡萄酒。在瓶內(nèi)密封24h后，有泡沫出現(xiàn)，汁液析出，葡萄皮浮起，每天用勺子攪動兩次，將葡萄皮壓到汁液中，讓它充分浸泡。 40mg/l：1L葡萄酒里需要加入6%。 將晾干的葡萄用粉碎機粉碎（用手捏碎）。：量取50mL酒樣，移入圓底燒瓶中，加入100mL蒸餾水，連接酒精蒸餾裝置，加熱蒸餾，直到蒸出的液體大約100毫升時，用蒸餾水定容至100mL，然后用酒精計測定酒精度，并且還要測定相應(yīng)的溫度，記錄溫度和酒精度。四、思考與練習(xí) 如何確定紅葡萄酒的皮渣分離時間？試驗八、葡萄酒發(fā)酵結(jié)束的理化指標(biāo)的測定一、 實驗?zāi)康牧私馊绾未_定葡萄酒酒精發(fā)酵的結(jié)束，同時對葡萄酒進行分離和封裝，轉(zhuǎn)入后發(fā)酵階段。8. 下膠與過濾，自然澄清半年后，用明膠下膠，通過下膠實驗確定用量(520g/hL)，然后用澄清板過濾。：每天測兩次比重、溫度，并定期用木柄壓“帽”、用冷水噴淋或在空調(diào)室內(nèi)控溫至26～30℃。按照20mg/L稱取果膠酶，在40℃左右活化10分鐘。 實驗七、干紅葡萄酒發(fā)酵的監(jiān)控一、實驗的目的掌握干紅葡萄酒釀造中發(fā)酵的監(jiān)控方法及相關(guān)的操作要求。：取20粒果實，洗凈擦干，撕下果皮并用吸水紙擦凈皮上所帶果肉及果汁，然后剪碎，(1 mol/L鹽酸 : 95％乙醇 = 15：85)50ml浸泡，浸泡20小時左右，然后測定540nm下的吸光度，計算果皮色價(XA x 10)/W (XA——吸光度，W——果皮重量g)。計算：在發(fā)酵結(jié)束后還需要再進行出汁率的測定?？呻S機取5 l0穗果實，裝入塑料袋于冰壺中，迅速帶回實驗室分析。同時簡單了解葡萄酒釀制的工藝原理。測濾液酒度，計算100g原料出酒率。待酵母活化后，按10%將其接種到裝有原料的三角瓶中。計算出實際實驗發(fā)酵液的產(chǎn)酸量。此時應(yīng)扣瓶使之充分通氣并散熱，測pH；再培養(yǎng)72h使瓶內(nèi)長滿豐盛的孢子即可出料，測pH。三、實驗材料及用品樣品：霉?fàn)€的桔皮菌種：黑曲霉（Aspergillus . niger）IFFI 2315培養(yǎng)基和試劑：（1） 酸性蔗糖培養(yǎng)基 蔗糖15% % % MgSO4?7H2O % 用鹽酸調(diào)pH≤ 121℃滅菌20min（2）固體發(fā)酵培養(yǎng)基 米糠:麩皮=2:1，65%水分（45ml:50g），121℃滅菌30min（3） NaOH溶液，1M鹽酸 （4）%酚酞指示劑：，溶于100ml 95%乙醇中器皿：白瓷托盤，保鮮膜，切刀，菜板，培養(yǎng)皿（帶濾紙），250ml三角瓶，搖床，恒溫培養(yǎng)箱，滅菌鍋，酸堿滴定管，紗布，牛皮紙四、實驗步驟（一）深層液體發(fā)酵菌種分離：取霉?fàn)€桔皮（）放入10ml三角瓶中，振蕩3～5min，然后用水稀釋5～10倍；菌種純化：～1ml放入酸性培養(yǎng)基上（稀釋液：培養(yǎng)基=1：10），搖勻，傾倒在平皿中的濾紙上，25℃培養(yǎng)2～3d即有菌落產(chǎn)生；發(fā)酵：將培養(yǎng)出的霉菌接種入液體酸性蔗糖發(fā)酵培養(yǎng)基中（25ml/250ml三角瓶），30℃搖床培養(yǎng)2～3d，過濾收集發(fā)酵液。適合于固體發(fā)酵法生產(chǎn)檸檬酸的原料諸如：甘薯渣、木薯渣、蘋果渣和甘蔗渣等。工業(yè)上的好氧發(fā)酵發(fā)基本上有三種，即表面發(fā)酵、固體發(fā)酵和深層發(fā)酵。寫出品嘗體會。然后換成中倍鏡觀察，繪制分離菌的形態(tài)圖，注明各部位名稱。3. 觀察平板上的菌落形態(tài)，用接種環(huán)調(diào)取霉菌菌落的孢子或菌絲體于新鮮的馬鈴薯蔗糖瓊脂平板上，再進行劃線培養(yǎng)，直至獲得純培養(yǎng)，即平板上只有一種霉菌的菌落或菌苔。接入種曲釀制：將冷卻到35℃左右的米飯，按干糯米重量換算接種量，%的經(jīng)粉碎的根霉曲與米飯拌勻，盛于廣口培養(yǎng)瓶中，飯粒搭成中心下陷的喇叭形凹窩，以利于出汁，飯面均勻撒上少許曲粉，用封口膜及牛皮紙覆蓋于廣口培養(yǎng)瓶表面，用線包扎后置于30℃溫箱保溫培養(yǎng)48h即可食用。四、操作步驟（一）甜酒釀的制作 酒 藥 洗米 蒸飯 淋水 降溫 落缸搭窩 發(fā)酵 甜酒釀浸米與洗米：選擇優(yōu)質(zhì)新鮮糯米，淘洗干凈后浸泡過夜，使米粒中的淀粉粒子吸水膨脹，便于蒸煮糊化，清水沖洗至水清亮，撈起瀝干。要初步學(xué)會和掌握釀制方法并不困難，從微生物學(xué)的觀點來看，釀制的關(guān)鍵在于：要有優(yōu)質(zhì)的酒釀種曲，即種曲中應(yīng)含有糖化率高的優(yōu)質(zhì)根霉、毛霉孢子或菌絲體；應(yīng)選擇優(yōu)質(zhì)的糯米作原料；嚴格無菌操作規(guī)程，盡量避免雜菌污染；合理控制釀制條件等。進一步掌握微生物的分離、培養(yǎng)等基本方法和無菌操作技術(shù)。 ≤90致病菌（指腸道致病菌和致病性球菌）不得檢出五、實驗報告將各批混菌發(fā)酵的酸奶品評結(jié)果記錄于下表中。 保加利亞乳桿菌在發(fā)酵的初期分解酪蛋白而形成氨基酸和多肽，促進了嗜熱鏈球菌的生長，隨著嗜熱鏈球菌的增加，酸度增加，抑制了嗜熱鏈球菌的生長。若采用兩種菌株混合培養(yǎng)，則含菌量?？杀对?。分離后，置于37℃下培養(yǎng)以獲得單菌落。⑼貯存 產(chǎn)品的貯存溫度為2℃~6℃。酸奶質(zhì)量的評定以品嘗為標(biāo)準(zhǔn)，通常有色澤、凝塊狀態(tài)，表層光潔度、酸度及香味、無異味等各項指標(biāo)。⑹培養(yǎng) 把接種后的發(fā)酵瓶置于4042℃溫箱中培養(yǎng)412h。⑵裝瓶 在250ml的酸奶發(fā)酵瓶中裝入牛奶200ml，裝瓶量80%。由于酸奶中含有乳酸菌的菌體及代謝產(chǎn)物，因而對腸道內(nèi)的致病菌有一定的抑制作用；對人體的腸胃消化道疾病也有良好的治療效果。學(xué)習(xí)并掌握從酸奶中分離和純化乳酸菌的方法。四、實驗中出現(xiàn)的問題及討論 配制培養(yǎng)基操作過程中的問題。二、儀器設(shè)備及原材料高壓滅菌鍋，250ml三角瓶，紗布，牛皮紙，瓊脂，其他化學(xué)藥品三、常用培養(yǎng)基的配方及制備細菌常