【正文】 r: Environment Engineering Grade: 2020 Student’s Name: Yongfu Liu Student No.: 09222069 Tutor: Lecturer Rui Hu Finished by May, 2020 畢業(yè)論 文 原創(chuàng)性聲明 本人所呈交的畢業(yè)論文 是我在導(dǎo)師的指導(dǎo)下進行的研究工作及取得的研究成果。 作者簽名： 日期： 畢業(yè)論文 授權(quán)使用說明 本論文 作者完全了解瓊州學(xué) 院有關(guān)保留、使用畢業(yè)論文的規(guī)定，學(xué)校有權(quán)保留論文 并向相關(guān)部門送交論文的電子版和紙質(zhì) 版。 作者簽名： 指導(dǎo)教師簽名： 日期： 日期： 劉永福 畢業(yè)論文 答辯委員會 (答辯小組 )成員名單 姓名 職稱 單位 備注 秦玉春 教授 理工學(xué)院 主席（組長） 李麗蓉 講師 理工學(xué)院 薛長風(fēng) 副教授 理工學(xué)院 王玉杰 講師 理工學(xué)院 瓊州學(xué)院本科生畢業(yè)論文 摘 要 生化需氧量 (BOD)是監(jiān)測水體中可生物降解的有機物含量的一個重要參數(shù) ,是目前最常用的水體有機污染綜合指標(biāo)之一 。 而日益得到廣泛應(yīng)用的微生物傳感器法能夠快速 、 準(zhǔn)確測定水體中 BOD,并可實現(xiàn)在線連續(xù)測定 ,更加適應(yīng)于現(xiàn)代環(huán)境監(jiān)測和管理的要求 。 本文綜述了微生物固定化方法在各類廢水處理中的研究現(xiàn)狀 ,對固定化方法進行了分類 ,并對其性能作了比較 。 1 ℃ , oxygen consumption of 5 days as the representative, called the 5 day biochemical oxygen demand, referred to as BOD5. But the method is plicated, timeconsuming, accuracy and reproducibility is poor, unable to meet the rapid determination of present environmental monitoring requirements. It has been widely used in microbial sensor method can fast, accurate determination of BOD in water, and can realize continuous online measurement, more suitable for modern environmental monitoring and management requirements. In this paper, the research progress and application of BOD microbial sensor technology. Analysis of the principle of microbial sensor and its advantages in measurement in water, has focused on its core part of biofilm research status and problems in the process of preparation. According to the research status of microbial membrane, to the breeding and the immobilized method chosen as the key research of this experiment. This paper summarized the present situation of research on microbial immobilization method in all kinds of waste water disposal, the immobilization methods are classified, and pared its performance. The Debaryomyces hansenii based biosensor is characterized by the highest sensitivity and, accordingly, the minimal lower limit of linear range. Thus, the Debaryomyces hansenii biosensor has the best characteristics of those tested. Response Surface Methodology (RSM) was used to investigate the effects of pH, Temperature and concentration buffers on the results. A 33 factorial design was performed to optimize the conditions. The experimental results showed that the optimal conditions are: pH of , temperature 20℃ and concentration buffers 40 mmol/L, and the small relative error of 1% between biosensor and BOD5. This study 瓊州學(xué)院本科生畢業(yè)論文 indicates that Debaryomyces hansenii based biosensor can be used as a reliable method to determine BOD in seawater, the linear fluctuant coefficients (R2) in the range of ~422 mg/dm3 when a glucose/glutamate BOD standard was applied. Such device provides a lowcost, easyoperated, fastresponse, sensitive and reliable method to measure BOD in seawater, and also is suitable for online measurements. Keywords: Biochemical Oxygen Demand(BOD)。 Dissolved Oxygen Eletrode瓊州學(xué)院本科生畢業(yè)論文 目 錄 第一章 緒論 ........................................................ 1 問題的提出 ...................................................... 1 傳統(tǒng)測量方法存在的問題 ...................................... 1 BOD 微生物傳感器的產(chǎn)生 ...................................... 1 課題研究的目的和意義 ............................................ 2 課題研究的內(nèi)容 .................................................. 3 第二章 實驗材料和方法 .............................................. 4 材料 ......