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單孢菌(絳紅小單孢菌)、白黃鏈霉菌、白色鏈霉菌、抗生鏈霉菌、雙重輪絲鏈霉菌、產(chǎn)色鏈霉菌、燼灰鏈霉菌、天藍(lán)色鏈霉菌、滅蚊鏈霉菌、紅霉素鏈霉菌、青色鏈霉菌、球孢鏈霉菌、淺灰鏈霉菌、灰色鏈霉菌、吸水鏈霉菌、淡紫灰鏈霉菌、黃色長孢鏈霉菌、藤黃色鏈霉菌、細(xì)黃鏈霉菌、黑化鏈霉菌、玫瑰色鏈霉菌、華美鏈霉菌、嗜熱鏈霉菌、委內(nèi)瑞拉鏈霉菌、紫色直絲鏈霉菌、紫色鏈霉菌、綠色鏈霉菌 13. Wort Agar (麥芽汁瓊脂) Dilute the world (without hop) to 12 Brix. Add 15g agar into 1000ml of the diluted word..Melt the agar by heating, then distribute the medium into tubes. Autoclave at 110 for 30 minutes. (將發(fā)酵啤酒的原料(未加酒花),稀釋至 12 柏林,加瓊脂 15 克,溶化后分裝。 ) 19. Filter Paper Medium (濾紙培養(yǎng)基) ( NH4) 2SO4 1g KH2PO4 1g NaCl Distilled water (蒸餾水) 1000ml A strip of filter paper (濾紙條) 6ⅹ 1cm (厘米) 20. Soybean Cake Meal Agar (黃豆餅粉瓊脂 ) 10% infusion of soybean cake meal (10%黃豆餅粉 浸汁 ) 1000ml Glucose (葡萄糖 ) 10g NaCl 21. Sawdust, Wheatbran Medium (木屑、麩皮培養(yǎng)基) Sawdust of broad leaf tree 75g Wheat bran 25g Preparation : Mix these two ingredients well . Add appropriate water and blend (the amount of water added may be becided by kneading with fingers ). Fill the mixture into broad mouth of bottles . Stick a hole on the filling, put the cotton pulg in bottle mouth . 殼斗科植物或闊葉樹的木屑 75 克 麩皮 25 克 制法:加水適量充分混勻,水量以手捏時指間有水珠為宜,裝瓶并壓緊,在中心處扎一小洞,堵上棉塞, 20 磅滅菌 1 小時。 ) 25. Rice Medium (米飯培養(yǎng)基 ) Fill 10g of rice into a 50ml of Erlenmeyer flask. Add 2030ml water. Autoclave at 121℃ for 30 minutes (or fill 2g of rice into a 15ⅹ 150mm tube). Add 6 ml of water. Autoclave at 121℃ for 30 minutes. (取大米 10 克,入 50 毫升椎形瓶中,水 2030 毫升于 15 磅滅菌 30 分鐘即成,或取大米 2 克,裝入 15ⅹ 150 毫米試管中,加水 6 毫升,于 15 磅滅菌30 分鐘。 7H2O Agar (瓊脂) 20g Distilled water (蒸餾水) 1L pH 31. Yeast Sporulation Medium (酵母菌產(chǎn)生子囊孢子培養(yǎng)基 ) (1) Kleyn Medium KH2PO4 % K2HPO4 % Sodium acetate ( 醋酸鈉 ) % Glucose (葡萄糖 ) % NaCl % Peptone (蛋白胨 ) % Biotin (生物素 ) 2μg% Agar ( 瓊脂 ) 2% Trace element solution (混合鹽類 ) 1ml/100ml [Note]: Trace element solution (混合鹽類溶液): %, NaCl %, %, %, % (2) Gorodkowa Medium Glucose (葡萄糖 ) % Peptone (蛋白胨 ) 1% NaCl % Agar (瓊脂 ) 2% (3) McClary Medium Glucose (葡萄糖 ) % KCl % Yeast juice (酵母汁 ) % Sodium actate (醋酸鈉 ) % Agar (瓊脂 ) % 32. BPY Medium (BPY 培養(yǎng)基 ) Beef extract (牛肉膏 ) 5g Prptone (蛋白胨 ) 10g NaCl 5g Yeast extract (酵母膏 ) 5g Glucose (葡萄糖 ) 5g Agar (瓊脂 ) 12% Distilled (蒸餾水 ) 1000ml pH 適用范圍:大腸埃希氏菌(大腸桿菌)、惡臭假單胞菌 33. LB Medium (LB 培養(yǎng)基 ) Yeast extact (酵母膏 ) 5g Peptone (蛋白胨 ) 10g NaCl 10g Agar (瓊脂 ) 12% Distilled water (蒸餾水 ) 1000ml pH 適用范圍:大腸埃希氏菌(大腸桿菌) 34. Penssay Broth (Penssay 肉湯 ) Beef extract (牛肉膏 ) Yeast extract (酵母 膏 ) Tryptone (胰蛋白胨 ) 5g NaCl Glucose (葡萄糖 ) 1g Distilled water (蒸餾水 ) 1000ml pH 35. Blond Agar (血瓊脂培養(yǎng)基 ) Peptone (蛋白胨 ) 10g Glucose (葡萄糖 ) 2g NaCl 5g NaH2PO4 Sodium citrate (檸檬酸鈉 ) 5g Brain infusion (腦浸汁 ) 800ml Pig heart infusion (豬心浸 ) 200ml Sleep Blood (羊血 ) 10% pH [Note]: Preparation of pig brain and pig heart infusion: ( 1) Smash up 100g fresh pig heart ( without fat and blood vessel) .Add distilled water up to 1000ml。id medium for cellulosedeposing bacteria: Carboxymethyl Cellulose 2% Agar 2%. (斜面培養(yǎng)基去濾紙條用 2%瓊脂及 2%羧甲基纖維素鈉 CMCNa ) 37. Clostridium Pasreurianum Synthetic Medium (巴氏梭狀芽孢桿菌合成培養(yǎng)基) Glucose (葡萄糖) 1% MnSO4 % KH2PO4 % FeSO4 % K2HPO4 % Yeast extract(酵母膏) % % Peptone (蛋白胨 ) % NaCl % CaCO3 % Distille water (蒸餾水 ) 100% pH 38. Yeast ExtractMedium Extract Agar Ⅱ (酵母膏、麥芽膏瓊脂 Ⅱ ) Yeast extract (酵母膏) 4g Mall extract (麥芽膏) 10g Glucose (葡萄糖) 4g Agar (瓊脂) 17g Distilled water (蒸餾) 1000ml pH 39. Oatemeal Agar (燕麥粉瓊脂) Oatemeal( infusion) [燕麥粉浸液 ] 20g Agar (瓊脂) 18% Trace element solution (跡量鹽溶液) 1ml Distilled water up to (用蒸餾水補充至 1000 毫升) 1000ml pH [Note]: Trace element solution (跡量鹽溶液 ): Distilled water (蒸餾水 ) 100ml 40. Calcium Malate Agar (蘋果酸鈣瓊脂 ) Calium malate ( 蘋果酸鈣 ) 10g K2HPO4 NH4Cl Glycerol (甘油 ) 10g Agar (瓊脂 ) 15g Distilled water (蒸餾水 ) 1000ml 41. Thiobacillus Ferrooxidans Medium (氧化亞鐵硫桿菌培養(yǎng)基 ) (NH4)2SO4 KH2PO4 KCl Ca(NO3) 50g Distilled water (蒸餾水 ) 1000ml pH 42. Thiobacillus Thiooxidans Medium (氧化硫硫桿菌培養(yǎng)基 ) (NH4)2SO4 KH2PO4 34g CaCl2 (anhydrous) [ 無水 ] Distilled water (蒸餾水 ) 1000ml pH 43. Plasma Substitute Medium (代血漿培養(yǎng)基 ) Sucrose (蔗糖 ) % Peptone (蛋白胨 ) % KH2PO4 % % pH 44. Skim Milk Medium (脫脂牛奶培養(yǎng)基 ) ATCC 183 脫脂牛奶 100g 去離子水 1000ml 適用范圍:保加利亞乳桿 菌、嗜熱乳酸鏈球菌 45. Pea Agar (豌豆瓊脂 ) Glucose (葡萄糖 ) 1% Peptone (蛋白胨 ) % NaCl % Agar (瓊脂 ) % Pea infusion (counted by NH2) [豌豆浸汁 (以 NH2 計 )] 1214 mg∕100 pH (before sterilization) [消前 ] [Note]: Preparetion of pea infusion (豌豆浸汁的制法 ) : Preparetion 900g of pea. Put them into a 5L bottle, and add 3L of tap water. Adjust pH to by using of sulphuric acid. Add 30ml of chloroform for antisepsis. Plug with rubber stopper and bind up the bottle mouth with gauge in order to avoid that the stopper fall off due to gas pressure. Place the bottle at 37℃ for 710 days. Filter the infusion, then boil the filtrate to escape the chloroform. Filter again Distribution and autoclave at 120℃ for 30 minutes. Store at cool c