紅細(xì)胞滲透脆性實(shí)驗(yàn)(文件)
【正文】 been used in screening for thalassemia.2. Either heparinized or defibrinated blood is suitable for osmotic fragility studies. 3. It is usually desirable to determine the osmotic fragility both of the freshly drawn blood and of autoincubated blood. For the latter, 2 ml of the sterile blood is placed in a sterile 5ml screwcap vial and incubated for 24 h at 37℃. A series of solutions containing phosphatebuffered sodium chloride solutions equivalent in osmolarity from to % NaCl are dispensed into tubes. A onehundredth volume of blood is added to each tube, as well as to a tube containing distilled water, which serves as the blank. After being allowed to stand at room temperature for 60 min, the tubes are centrifuged and optical density of the supernatant is read at 540 nm.4. The percent hemolysis in each tube is calculated as follows:% hemolysis = (ODsODB)100 OdoWhere ODs is the optical density in a supernatant, ODB is the optical density of the % saline supernatant, and ODo is the optical density of the distilled water tube. 5. The normal range of values is presented as follows:NaCl, % Lysis, %Fresh Incubated 95100 97100 85100 9099 75100
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