【正文】
ose isomerase) ? 核酮糖 5磷酸差向異構(gòu)酶 ( ribulose5phosphate eimerase)。 ? 第二, AraC既 可充當(dāng)阻遏物,也可作為激活劑。在標(biāo)準(zhǔn)的遺傳學(xué)圖譜上, araBAD基因簇從啟動子 PBAD開始向右進(jìn)行轉(zhuǎn)錄,而 araC基因則是從Pc向左轉(zhuǎn)錄。這樣,阿拉伯糖的誘導(dǎo)作用就可以解釋為阿拉伯糖與 Pr的結(jié)合,使 Pr離開它的結(jié)合位點(diǎn),然后,產(chǎn)生大量的 Pi,并與啟動子結(jié)合。 ? araC的表達(dá)受到自身產(chǎn)物 AraC的自動調(diào)控。 ? 阿拉伯糖作為 ,可以誘導(dǎo) ara操縱子的轉(zhuǎn)錄。阿拉伯糖與AraC結(jié)合似乎改變了 AraC同源二聚體化特性和促進(jìn)了 AraCCAP復(fù)合物的形成。 ? When the araC protein is bound to the operator araO1, transcription from PC is prevented. ? When arabinose levels are low, the AraC protein acts as a repressor and binds to two operator sites, araO1 and araO2, as well as to araI. ? Binding to araO1 inhibits transcription of the araC gene itself. Thus, araC is autoregulated at the level of its own transcription. AraC molecules bound to araO2 and araI interact with each other to form a DN