色譜分離法(文件)
【正文】 密 堆 積 檢 查 好 管 柱 是 否 暢 通 1 2 Gel Gel 14 裝置示意圖 15 16 17 凝膠過濾色譜圖 18 凝膠過濾色譜標準曲線 19 酶分離純化的方案設(shè)計 The following information is known about four proteins: Protein Mr pI Subunits A 13,100 1 B 14,200 1 C 35,500 1 D 200,000 4 identical, 50,000 each 20 a. Sketch the elution profile expected if this mixture is run on a Sephadex G100 gel filtration column run in 50 mM phosphate buffer, pH 6. Label the peaks. b. Sketch the gel obtained from running these proteins on an SDS polyacrylamide electrophoresis gel at pH , (after staining and destaining). c. What predictions can you make about the results of a native PAGE at pH ( state any assumpti
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