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. pHdependent fluorescence and absorbance of FluorPEG/SWNT. Absorption curves of FluorPEG/SWNT at various pHs in the (a) fluorescein region (dashed line marks the peak at pH 12) . Figure 3. (b) SWNT region (curves are displaced for clarity). Figure 3. (c) Corresponding fluorescence emission spectra (236。excitation ) 495 nm). Figure 3. (d) Comparison of emission peak intensity dependence on pH of free FluorPEG and FluorPEG/SWNT with the same FluorPEG concentration. Figure 4. Cellular uptake of FluorPEG/SWNT. (a) Confocal fluorescence image of BT474 cells incubated with FluorPEG/SWNT. Figure 4. (b) Fluorescence intensities of large population of cells treated with FluorPEG/SWNT vs untreated control cells by flow cytometry. Figure 4. (c) MicroRaman image of the cells incubated with FluorPEG/SWNT. Figure 4. (d) Representative Raman spectra showing the presence and absence of the SWNT Gband from FluorPEG/ SWNTincubated cells and untreated control cells, respectively. Preparation of polymercoated mesoporous silica nanoparticles used for cellular imaging by a ‘‘graftfrom’’ method 4 Yang Yang, Xuehai Yan, Yue Cui, Qiang He, Dongxiang Li,ac Anhe Wang, Jinbo Fei and Junbai Li etc J. Mater. Chem., 2022, 18, 5731–5737 | 5731