基于電活性納米復合材料構(gòu)建生物傳感的研討-wenkub
2023-04-22 03:22:26 本頁面
【正文】 face, incorporating the distinctive features of nanomaterials and biomolecules to exert a synergistic effect, contributes a lot to the development of biosensors. With emphasis on the contruction of nanofilms and nanobiomolecule posite interfaces, as well as their application in electrochemical biosensors, this article has carried out the following works:1. A novel amperometric immunosensor has been developed by selfassembling gold nanoparticles (Au NPs) onto a toluidine bluebranched chitosan (CHITTB) modified electrode for the sensitive determination of hepatitis B surface antigen (HBsAg) as a model protein. The formation of CHITTB posite film not only effectively avoids the leakage of TB and retains its electrochemical activity, but also enhances the conductivity and chargetransport properties of the posite. Further adsorption of Au NPs into the CHIT matrix provides both the interactive sites for the immobilization of HBsAb and a favorable microenvironment to maintain the activity of the HBsAb, in addition prevents leakage of HBsAb molecules from the CHITTB/Au NPs/HBsAb film structure efficiently. The morphologies and electrochemistry of the formed nanoposite film were investigated by using scanning electron microscopy and electrochemical techniques including cyclic voltammetry and electrochemical impedance spectroscopy. The HBsAg concentration was measured through the decrease of amperometric responses in the corresponding specific binding of antigen and antibody. The decreased voltametric values were proportional to the HBsAg concentration in the range of 1 to 200 ng mL1. The detection of HBsAg levels in five sera obtained from hospital showed acceptable . A novel electrochemical immunosensor for sensitive
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