化學(xué)誘變劑ems篩選越橘莖尖抗旱突變體的初步研究(已修改)
2025-06-19 17:09 本頁面
【正文】 化學(xué)誘變劑EMS篩選越橘莖尖抗旱突變體的初步研究陳凌，張文玲，張敏，李政，李凌西南大學(xué) 園藝園林學(xué)院，重慶 400716摘要: 本實(shí)驗(yàn)以兩個(gè)品種越橘繼代苗莖尖為試材，用甲基磺酸乙酯(EMS)對(duì)其莖尖進(jìn)行處理后，外植體再生率和再生株生根率比對(duì)照均有所下降。M1代植株經(jīng)干旱脅迫后進(jìn)行初步篩選后，過氧化氫酶（CAT）、超氧化物歧化酶(SOD) 作者簡(jiǎn)介：陳凌（1985），男，重慶潼南人，碩士研究生，主要從事園林植物遺傳育種與生理研究通訊作者：李凌，教授，碩士生導(dǎo)師。、過氧化物酶(POD)活性增強(qiáng)，丙二醛(MDA)含量減少，對(duì)干旱環(huán)境表現(xiàn)出一定的抗性。%EMS誘變處理后可能存在抗旱性較強(qiáng)的突變植株。關(guān) 鍵 詞: 甲基磺酸乙酯； 越橘； 誘變； 抗旱突變體中圖分類號(hào)：The initial study on screening drought resistance mutants of blueberry shoots with EMS mutagenesis CHEN Ling，Zhang Wenling，Zhang Min，LI Zheng, LI Ling(School of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716,China)Abstract: The meristem of blueberry culture was treated by EMS, both the shoot induction rate and the rooting rate decreased more than that of control. Drought tolerant variants were selected from the M1 generation plants, then the activity of SOD, CAT, POD enhanced and the content of MDA decreased which means that the regenerated plants’ ability of drought resistant was improved. The strongest drought resistant mutant plants were obtained at the treatment of %EMS.Key words: EMS。 blueberry。 indu
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