【正文】 xx 大學(xué)學(xué)士學(xué)位論文 I 基于石英晶體的生物傳感器的研究 摘要 凝血因子的分析在臨床檢查中被廣泛應(yīng)用，目前所有的檢測方法都是以凝集反應(yīng)為基礎(chǔ)，通過血液凝集過程中反應(yīng)體系的粘度、密度等的變化來判斷血液凝集反應(yīng)的終點。血液凝集終點的判斷可通過自動化儀器或肉眼觀察。肉眼觀察，秒表計時，檢測結(jié)果誤差大，重復(fù)性較差；自動化儀器結(jié)果準(zhǔn)確、快速、重復(fù)性好，但儀器昂貴、檢測成本高，故需尋找一種靈敏、簡單、精確測量、成本低廉的新檢測方法。 壓電石英諧振測量技術(shù)能夠準(zhǔn)確地測量出微觀反應(yīng)過程中的微小變化并轉(zhuǎn)化為可以定量檢測的頻率信號，具有亞 ng 級別的質(zhì)檢 能力，從而為獲取反應(yīng)體系豐富的在線實時信息提供了一種簡單、快捷的手段。我們將血液凝集反應(yīng)與之結(jié)合，通過適當(dāng)?shù)纳飳W(xué)處理和結(jié)構(gòu)設(shè)計，構(gòu)建出一個基于壓電石英晶體傳感技術(shù)的血漿凝血因子檢測系統(tǒng)?；趩纹瑱C，采用等精度測頻對壓電石英生物傳感器的硬件結(jié)構(gòu)進(jìn)行頻率計數(shù)設(shè)計。以壓電石英晶體傳感器振蕩頻率檢測系統(tǒng)為平臺，建立了壓電傳感器頻率動態(tài)響應(yīng)模型。 結(jié)果表明：本檢測系統(tǒng)提高了凝血因子檢測的方便性、精度和一致性，具有精度高和成本低等優(yōu)點，有廣闊的推廣前景。 關(guān)鍵詞 壓電石英晶體；生物傳感器； 檢測 xx 大學(xué)學(xué)士學(xué)位論文 II Studies of Biosensor Based on Piezoelectric Quartz Crystal Abstract The analyses of coagulation factor has been widely used in the clinical examination, now all kinds of detection methods based on agglutination, the end of the blood agglutination is judged by the viscosity and density of reaction system during blood agglutination. The end of the blood agglutination is judged by automated equipment or visual observation. The error of detection is big and poor repeatability by visual inspection and stopwatch time. The results obtained by automation equipment is accurate, rapid and reproducible, but the equipment is expensive and testing cost is high, so a sensitive, simple, precise measurement, lowcost new detection method is developed. Piezoelectric quartz resonator measurement technique can accurately measure the small change in the microcosmic reaction process and can be transformed into quantitative detection frequency signal, with subng level quality inspection capabilities, so a simple and fast method is provided for obtaining the abundant online realtime information of response system. Combined with the blood agglutination, through appropriate biological processing and structural design, we propose a plasma coagulation factor detection system sensor based on piezoelectric quartz crystal technology. The frequency count of hardware structure of piezoelectric crystal biosensor is designed based on microcontroller, using equal precision frequency measure. Using piezoelectric quartz crystal sensor oscillation frequency detection system for the platform, we establish piezoelectric sensor frequency dynamic response model. The results showed that: the detection system improve convenience, consistency, and accuracy of detection coagulation factor, with low cost, high accuracy and widely popularizing prospect. Keywords Piezoelectric Quartz Crystal。 Biosensor。 Detection xx 大學(xué)學(xué)士學(xué)位論文 III 目錄 摘要 …… ....................................................................................................................... I Abstract ........................................................................................................................ II 第 1 章 緒論 ................................................................................................................ 1 課題背景 ........................................................................................................... 1 壓電傳感器的發(fā)展史 ....................................................................................... 2 壓電生物傳感器的研究進(jìn)展 ........................................................................... 3 論文研究內(nèi)容 ................................................................................................... 4 第 2 章 壓電生物傳感器與凝血因子檢測系統(tǒng) ........................................................ 6 硅材料 ............................................................................................................... 6 壓電石英晶體傳感器的基本原理 ................................................................... 6 壓電石英晶體 ............................................................................................ 6 切型及金屬電極的選擇 ............................................................................ 8 Sauerbery 方程 ............................................................................................ 8 振蕩電路 .................................................................................................... 9 液相壓電傳感理論 .................................................................................... 9 凝血因子檢測系統(tǒng) ......................................................................................... 12 凝血因子檢測方法 .................................................................................. 12 石英振子的加工制備 .............................................................................. 13 壓電傳感器檢測池的設(shè)計 ...................................................................... 14 檢測儀的設(shè)計 .......................................................................................... 15 本章小結(jié) ......................................................................................................... 16 第 3 章 壓電石英傳感器頻率測量技術(shù)的實現(xiàn) ...................................................... 17 頻率的測量 ..................................................................................................... 17 等精度頻率的測量技術(shù) .......................................................................... 17 等精度頻率測量的基本原理 .................................................................. 17 等精度測頻的測量誤差 .......................................................................... 19 石英 晶體傳感器凝血因子 頻率檢測 .......................................................... 19 壓電石英晶體傳感器等精度頻率測量的硬件實現(xiàn) .............................. 19 壓電石英晶體傳感器等精度測頻的軟件實現(xiàn) ....................................... 25 本章小結(jié) ......................................................................................................... 26 第 4 章 壓電傳感器頻率動態(tài)響應(yīng)模型的建立 ...................................................... 27 凝血反應(yīng)體系粘度密度變化規(guī)律 ................................................................. 27 壓電石英晶體傳感器頻率變化與性質(zhì)的關(guān)系 ............................................. 28 粘度密度乘積變化與△ F 的關(guān)系 ........................................