《芯片技術(shù)徐翔麗》ppt課件-文庫(kù)吧
2025-04-18 04:41 本頁(yè)面
【正文】 芯片主要用來(lái)檢測(cè)蛋白質(zhì)的生物學(xué)活性。 二、根據(jù)檢測(cè)試劑類(lèi)型 抗體芯片、蛋白質(zhì)芯片、肽芯片、適配體芯片、小分子芯片等。為了檢測(cè)自身抗體，自身抗原也被也被用做檢測(cè)試劑，這一類(lèi)蛋白質(zhì)芯片叫做抗原芯片。 三、根據(jù)密度分類(lèi) 低密度蛋白質(zhì)芯片、高密度蛋白質(zhì)芯片。 四、根據(jù)樣本分類(lèi) 蛋白質(zhì)芯片能夠從不同的生物樣品中測(cè)出單一的蛋白質(zhì)，因此蛋白質(zhì)芯片又被稱(chēng)為細(xì)胞溶解物蛋白質(zhì)芯片、條件化培養(yǎng)基蛋白質(zhì)芯片、血清蛋白質(zhì)芯片、反相蛋白質(zhì)芯片等。 Quantitative analysis of human serum leptin using a nanoarray protein chip based on singlemolecule sandwich immunoassay Talanta 78 (2022) 608–612 We report a method for the quantitative analysis of human serum leptin, which is a protein hormone associated with obesity, using a nanoarray protein chip based on a singlemolecule sandwich nanoarray patterning of a biotinprobe with a spot diameter of 150 nm on a selfassembled monolayer functionalized by MPTMS on a glass substrate was successfully acplished using atomic force microscopy (AFM)based dippen nanolithography (DPN). Unlabeled leptin protein molecules in human serum were detected based on the sandwich fluorescence immunoassay by total internal reflection fluorescence microscopy (TIRFM). The linear regression equation for leptin in the range of 100 zM–400 aM was determined to be y = x + 80,382 ( R = ). The accuracy and sensitivity of the chip assay were clinically validated by paring the leptin level in adult ser
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