蛋白質(zhì)生化分離技術(shù)-文庫吧
2025-01-03 20:00 本頁面
【正文】 I Classification of bioseparation steps (5) ?New words and vocabulary ? Highresolution fractionaton (高分辯率分級） ? Harvesting (收獲，收割） ? Clarification （澄清） ? Enrichment （富集） ? Hazardous （危險） ? Integrate（集成） ? Intensification（集成，加強(qiáng)） ? Expanded bed adsorption （擴(kuò)張床） ? Homogenate （勻漿液） ? Fermentation broth（發(fā)酵液） IV Upstream and downstream processing (1) ? Minimize loss of the valuable biological product ? (1) Location and form of proteins in the cell ? (2) Separation of cellular debris and inclusion bodies （ Increasing the number of passes through the homogenizer） ? (3) Separating a biological product by different protocols ? (4) Understanding upstream and downstream conditions (harvesting time and cell wall strength and proteases) IV Upstream and downstream processing (2) ? Biological process development objective: ? (1) Product to the market in quickest time possible ? (2) meeting all possible safety requirements ? (3) process cost effective ? (4) reliable ? (5) safety factors particularly important during scaleup IV Upstream and downstream processing (3) ? Enhance the quantity and quality ? (1) continuous media sterilization ? (2) enhance mass transfer ? (3) gas analysis by mass spectrometry ? (4) puterized data (expert system) ? (5) search for novel techniques ? A microgravity separation (eliminate shear stress) ? B molecular imprinting polymers ? C foams separation IV Upstream and downstream processing (4) ? Separation under space microgravity ? (1) higher throughputs for electrophoretic separation ? (2) two phase partitioning under microgravity (mixing and demixing) ? (3) crystallization of proteins under microgravity (need weak interacting force when protein forming crystallization) ? (4) advantages and expense carefully analyzed on ? ground bioprocessing for different application IV Upstream and downstream processing (4) ? New Words and vocabulory ? Coarsegrade centrifuge (粗糙級別的離心） ? Harsher （苛刻的） ? Intractably (不聽話的） ? Debris (碎片） ? End
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