【正文】
ons are well established and usually require ES cells to be grown on an inactive feeder cell layer or on gelatincoated plates with Leukemia Inhibitory Factor (LIF) in the culture medium. 一般的培養(yǎng)條件建立起來后,通常需要干細胞在不活躍的環(huán)境生長,或 在凝膠包被的帶有殺傷細胞抑制因子的培養(yǎng)基中生長. Mouse ES Basal Medium has been optimized to grow and maintain undifferentiated mouse embryonic stem cells. 小鼠干細胞基礎(chǔ)培養(yǎng)基優(yōu)化后可用作維護和生長未分化的小鼠干細胞. The medium may be used with the addition of Foetal Bovine Serum (FBS) or with any serum replacement designed for mouse ES cells. 培養(yǎng)基可以和牛血清一起使用或可以和任何為小鼠干細胞培養(yǎng)專門設(shè)計的添加劑一起使用 The medium contains stable glutamine dipeptide, and is therefore stable for at least 18 months from production, when stored at