【正文】
s] response surface methodology。這些結(jié)論為提高喜樹葉黃酮利用率及其工業(yè)化生產(chǎn)提供了一定的理論參考。本實驗首先對可能影響喜樹葉黃酮提取的五個因素:料液比,提取次數(shù),乙醇體積分?jǐn)?shù),提取溫度,每次提取時間進(jìn)行單因素試驗,結(jié)果可以找出各因素與提取率的大致關(guān)系,并選擇了三個顯著影響因素即料液比,乙醇體積分?jǐn)?shù),提取溫度,以提取率為響應(yīng)值,進(jìn)行了經(jīng)典的三因素三水平BoxBenhnken 試驗設(shè)計,并通過DesignExpert軟件對實驗數(shù)據(jù)進(jìn)行分析,得到相關(guān)響應(yīng)面數(shù)據(jù)和分析結(jié)果。響應(yīng)面設(shè)計優(yōu)化喜樹葉黃酮提取工藝畢業(yè)論文目錄摘 要 IAbstract II第1章 緒論 1 喜樹 1 黃酮類化合物活性及生理作用 1 國內(nèi)外研究現(xiàn)狀 2 響應(yīng)面設(shè)計分析方法 2 實驗研究方法 3第2章 材料與方法 4 4 材料、藥品試劑及儀器 4 實驗材料 4 主要藥品與試劑 4 主要儀器與器具 4 5 樣品處理 5 相關(guān)溶液的配制 5 黃酮含量測定的標(biāo)準(zhǔn)溶液及標(biāo)準(zhǔn)曲線的繪制 5 黃酮的提取 6 黃酮含量測定方法 6 黃酮提取單因素試驗 6 料液比的影響 6 提取次數(shù)的影響 6 乙醇體積分?jǐn)?shù)的影響 6 提取溫度的影響 7 提取時間的影響 7 7 7 實驗分析 7第3章 結(jié)果與分析 8 8 單因素試驗結(jié)果與分析 8 料液比的對提取率的影響 8 9 乙醇體積分?jǐn)?shù)對提取率的影響 10 提取溫度對提取率的影響 11 提取時間對提取率的影響 12 響應(yīng)面設(shè)計試驗結(jié)果及分析 12 BoxBenhnken 設(shè)計方案及試驗結(jié)果 12 14 16 最佳工藝條件 17第4章 結(jié)論與討論 18附錄 19參考文獻(xiàn) 22致 謝 24響應(yīng)面設(shè)計優(yōu)化喜樹葉黃酮提取工藝摘 要 研究顯示,喜樹葉中黃酮類化合物含量相對豐富。為提高喜樹葉中黃酮類化合物的提取效率,本論文在以風(fēng)干喜樹葉為原料, 用乙醇作提取劑,在喜樹葉的黃酮提取工藝中, 將單因素試驗和響應(yīng)面實驗設(shè)計分析法應(yīng)用于回流提取喜樹葉黃酮工藝, 研究回流提取的條件及其對提取結(jié)果即黃酮的得率的影響,尋求各因素的綜合優(yōu)化條件。結(jié)果表明,乙醇回流提取喜樹葉黃酮的最佳工藝條件為1:40料液比,60%乙醇,溫度80℃,每次提取90 min,提取三次?!娟P(guān)鍵詞】響應(yīng)面法; 喜樹葉; 黃酮提取 IOptimization of Extraction Process of Flavonoids from Camptotheca acuminata leaves Using Response Surface MethodologyAbstractStudies have shown that the content of flavonoids in Camptotheca acuminata leaves is relatively abundant. In order to improve the extraction efficiency of flavonoids in Camptotheca acuminata leaves, studies of the paper used airdry Camptotheca acuminata leaves as raw material, ethanol as extraction agent. In this extraction process, the single factor experiment and response surface experimental design analysis method were applied to reflux extraction, to research reflux extraction conditions and their impact on the yield of flavonoids extraction results, and seek the optimal conditions. The single factor experiments first had five factors that may affect the yield of flavonoids extraction: solidliquid ratio, extraction times, ethanol concentration, extraction temperature and extraction time and the results had shown that the approximate relationship of the various factors and extraction rate, and three significant factors: solidliquid ratio, ethanol volume fraction, extraction temperature. Then the classic three factors and three levels BoxBenhnken experimental design was carried out to establish a quadratic regression model for the extraction efficiency of total flavonoids, and analysis of the experimental data was processed by the DesignExpert software. The optima