【正文】
青龍臥墨池、十八號牡丹、黑花魁、豆綠、金星雪浪、大胡紅、荷花紫、枯枝牡丹等,這在一定程度上反映出目前的牡丹組培研究工作還處于初級階段,研究的廣度有待加強。國內(nèi)組培苗還沒有形成商品苗批量生產(chǎn)。20多年來,中外學(xué)者對牡丹的組織培養(yǎng)進(jìn)行了大量的工作,均取得了不同程度的進(jìn)展。利用組培技術(shù)繁殖牡丹,有周期短,繁殖系數(shù)高,便于大規(guī)模生產(chǎn),因此,各種牡丹品種群的組培技術(shù)已經(jīng)成為主要的研究方向。牡丹傳統(tǒng)的繁殖方法有有性生殖中的種子繁殖,無性繁殖中的分株、壓條、嫁接繁殖等[1]。其花朵碩大,雍容華富,作為文明、和諧、繁榮、富強的象征,在國內(nèi)外有廣闊的文化市場需求。 tissue culture。關(guān)鍵詞:組織培養(yǎng); 牡丹; 植物STUDY ON TISSUE CULTURE TECHNIQUES FOR PEONYABSTRACTThe peony is China39。同時也指出整個操作過程中的主要問題所在:玻璃苗、褐化等一系列的影響因素。文中對牡丹組織培養(yǎng)近30年的研究進(jìn)展進(jìn)行了綜述,主要有:牡丹組織培養(yǎng)過程中外植體的選擇與消毒技術(shù)、培養(yǎng)基選擇的、培養(yǎng)條件、生根轉(zhuǎn)移培養(yǎng)等各個操作階段的技術(shù)要領(lǐng),包括牡丹品種、部位的選擇,培養(yǎng)基植物調(diào)節(jié)劑的添加量,培養(yǎng)環(huán)境中溫度、光照等條件的控制以及生根過程中促進(jìn)生根措施等。畢 業(yè) 論 文牡丹組織培養(yǎng)技術(shù)的研究概述牡丹組織培養(yǎng)技術(shù)的研究概述摘 要牡丹是我國名花,傳統(tǒng)繁殖方式不僅成苗周期長,而且出苗量少,質(zhì)量參差不齊,難以滿足生產(chǎn)需要。因此,用組織培養(yǎng)的方法對牡丹進(jìn)行繁育,成為牡丹商品化和產(chǎn)業(yè)化發(fā)展的必然趨勢。此外,對愈傷組織的誘導(dǎo),花粉和花粉培養(yǎng),胚培養(yǎng)等多種組培方法的研究也進(jìn)行了全面的總結(jié)。目前,牡丹的組織培養(yǎng)仍然處于研究階段,存在許多不完善的領(lǐng)域,通過對前人研究成果的總結(jié),為今后牡丹組織培養(yǎng)工作的進(jìn)一步開展提供借鑒,推進(jìn)牡丹組培技術(shù)的發(fā)展。s famous flowers, traditional breeding methods not only seedling period is long, and the emergence of the quantity is little, quality is uneven, it is difficult to meet the need of production. Therefore, using tissue culture method for breeding of peony, peony mercialization and industrialization has bee the inevitable trend of the development of. In this paper, the peony tissue culture in recent 30years were reviewed, mainly include: Peony explant selection and sterilization technology, selection of culture medium, culture conditions, the transfer of cultured rooting each operation stage of the technical essentials, including peony varieties, site selection, medium, plant growth regulator added in the culture environment, temperature, illumination control and during Rooting rooting measures. In addition, the induction of the callus, pollen and pollen culture, embryo culture and other culture research methods also undertook prehensive summary. At the same time also pointed out that the entire operation process of the main problems: the vitrification, browning and a series of factors. At present, the peony tissue culture is