生物技術(shù)藥物,12。stemcell-展示頁(yè)
2025-01-17 02:16本頁(yè)面
【正文】 e ? Screen for animals with the gene integrated into their germ line cells ? Selective breeding to establish new geic lines Mouse Trangenesis Methodology ? Retroviral vectors that infect cells of early stage embryos followed by implantation and selective breeding ? Microinjection of DNA into the nucleus of a fertilized egg followed by implantation and selective breeding ? Introduction of geically engineered embryonic stem cells into early stage embryos followed by implantation and selective breeding Mouse Transgenesis Retroviral Vector Method Mouse Transgenesis Retroviral Vector Method ? Efficient mechanism of integrating the transgene into the recipient host cell ? Can transfer only small fragments of geic information ( 8 Kb) ? Random insertion of transgene in host genome ? Potential for retroviral contamination in transgenic animal Mouse Transgenesis Microinjection Method Transgenic animals “ A little bit of this, and a little bit of that?” Mouse Transgenesis Microinjection Method ? Low efficiency of transgenesis ( 5%) ? Injected transgene integrates at random sites within the host genome ? Variable levels of expression ? Genes for selection not required Efficiency of Microinjection Mouse Transgenesis Embryonic Stem Cell Method Mouse Transgenesis Embryonic Stem Cell Method ? Efficient transfection in culture ? Positivenegative selection and enrichment of ES cells in culture ? Targeted insertion in host cell genome ? Targeted gene disruption and development of geneknockout strains Negative Selection Positive Selection Gene Knockout Transgenic Mice: Applications ? Model systems for human diseases and feasibility studies for production of therapeutic agents ? Mouse models for human diseases such as Alzheimer disease, arthritis, muscular dystrophy, tumorigenesis, hypertension, neurodegenerative disorders, and coronary disease Animal Cloning: To Clone, or not to Clone Dolly Geia Agriculture Education Curriculum Office July 2022 Pros: ? Cure human diseases ? Using animal ans ? Create animals that are disease resistant ? More consistent food products ? Save endangered species Cons: ? Public perception ? Use technology to clone huma
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