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翻譯 譯文題目 :生化的分析者 將 Olli C + D 使用在朝派酶學(xué)一年后的評價(jià) 學(xué)生姓名: 呂錦陽 學(xué) 號: 050921060 專 業(yè): 生物醫(yī)學(xué)工程 方 向: 醫(yī)療器械 指導(dǎo)教師: 但漢久 2021 年 12 月 29 日 咸寧學(xué)院本科畢業(yè)設(shè)計(jì)(論文):全自動(dòng)生化分析儀的維護(hù)與保養(yǎng) 1 Evaluation of the Olli C + D biochemical analyser after over a year of use in enzymology G. Baraton, D. Grafmeyer, A. Capuano, L. Richard and J. Sofia. Laboratoire automatisde Biochimie Clinique, (Professeur J. Bertrand), HopitalEdouardHerriot, Place d’Arsonval, 69374 Lyon Cedex 2, France To meet today’s clinical requirements, clinical biochemistry laboratories must be increasingly automated. The required criteria for an automatic analyser are reliability。 the ability to change chemical reactions easily and quickly。 it is possible to add serum and up to eight reagents distributed in one or several runs. Each analysis programme follows the sequence:l. Attain reaction temperature (25, 30, 37C). 2. Measure reagent (5 to 1000/al by steps of 1/.tl) and position. 3. Measure and add sample. 4. Mix(5 to 180 seconds). The photometer measures 330 x 520 x 740 mm and weighs 48 kg. It prises a puter, a thermal pri_nter and a thermostated measuring chamber (25, 30, 37C). The light source is a zenon lamp. A quartz optic fibre system allows the simultaneous measurement of 24 cuvettes. Filters have a bandwidth of 8 to 15 nm. The smallest readable volume is 500 /al for end point determinations, and 300/1 for kiic determinations. The photometer can be programmed by hand or by tape cassette for 15 analyses which may be made in end point mode, with or without blank measurement, or in kiic mode, with or without reagent or serum blanks. Kiics determinations are made using linear regression of 12 to 24 measurements in to 10 minutes. The results are printed with the name and units of the programmed analysis. Error messages are printed。 Miniken (Coultronic),Cobas BIO (Roche,) PA 800 (Vitratron), ACP 5040 (Eppendorf) On the Olli C, the time needed to reach the set temperature of 30C or 37C with an analysis volume of 550 ul(contained in the acrylic cuvette in the thermostated block)was followed by noting the decrease in the absorbance of the paranitrophenol solution. The temperatures of cuvette contents were also monitored with a Metrix temperature probe (HA 1159). Optical performance This was tested according to the procedure of Rand [5].Linearity and precision were tested using the following kits:UVTrol (Biomerieux France) and Holnicob (Biotrol France). Method All analyses were performed at either 30C or 37C. The instrument settings were those remended by the oxaloacetic transaminase (SGOT) and pyruvic transaminase (SGPT) were measured by the Wroblewski method at 37C or by the remended method of the SFBC at 30C with the Biomerieux kits. Alkaline phosphatase (ALP) was measured following the remended procedure of the German Society of Clinical Biochemstry (DGKC) at 37C. Accuracy, within day and day to day, and carryover were tested using low, medium and high concentration control sera spiked where necessary with animal enzymes. These control sera were furnished by a local association operating in biological measurement quality control. (Pro Bio. Qual, Lyon, France). Results and discussion Accuracy and precision of the Olli D sample processor The results are presented in Table 1. Precision expressed as the coefficient of variation for 24 determinations was never greater than % for the most important dilution (10 of drawing ink in 510 1 o