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【正文】 A 13,100 1 B 14,200 1 C 35,500 1 D 200,000 4 identical, 50,000 each 20 a. Sketch the elution profile expected if this mixture is run on a Sephadex G100 gel filtration column run in 50 mM phosphate buffer, pH 6. Label the peaks. b. Sketch the gel obtained from running these proteins on an SDS polyacrylamide electrophoresis gel at pH , (after staining and destaining). c. What predictions can you make about the results of a native PAGE at pH ( state any assumptions you might need to make about the % acrylamide in the gel ) 21 d. Sketch the elution profile of these proteins from a carboxymethyl cellulose ion exchange chromatography column, run at pH (with a salt gradient, if necessary)
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