【正文】 g my college years, with the systematic education, I obtained a solid academic background in chemistry and basic biology science especially in experiment skills as a preparation for future advanced research, which is also, the basis for me to do my current research project at the lab. Due to my outstanding experiment skills, in the third year of my undergraduate study, I was remended to the State Key Lab of Microbial Resource, Institute of Microbiology, Chinese Academy of Science, which only 2% the students can get in, to do research on the structure and character of a new family of DNABinding Protein from the Hyperthermophilic Archaeon Sulfolobus Shibatae(extreme environment). And independently I have been dedicating in the interaction between DNA and DNABinding Protein by Gel Retardation Assay, Nick Closure Assay and EMSA. This project provided me with not only considerable experiment experience including bacterial culture, expression of rebinant, and western blotting etc, but also how to plan an experiment, by consulting papers, seeking optimal conditions and selecting appropriate methods. Now my partners and I have made a great achievement of this project and we have a paper, “A Highly Conserved DNABinding Protein from the Hyperthermophilic Archaeon Sulfolobus Shibatae Affects DNA Supercoiling in a TemperatureDependent Fashion”, which will be published in the NUCLEIC ACIDS RESEARCH() in the nearer future. Furthermore, I have once, as a cooperator, helped other group investigate Archaeon ssh7 DNA binding protein and DNA interaction. With the experiment process, I am proficient in PCR and RTPCR, southern and northern blotting, cell insitu blotting, CsCl density gradient centrifuge, expression of rebinant protein, FPLC, protein purification and protein/enzyme activity assay, etc.In addition to academic studying and lab training, I benefit much from my college life. I joined the Green Life Association, one of the best student organizations in Peking University, and had been the minister of propagation for one year. The job has deepened my love for lives and improved my responsibility both for group and for the wellbeing of our earth. Moreover, all the manysided pleasant experiences and excellent records are a great treasure to me, giving me high confidence and independence.Why to go abroad to pursue .? I’m always thinking that the research of life sciences is not only interdisciplinary but also international. Besides the advanced technology, I hope to fuse the analytic way of the east and west. So I’d like to go abroad for advanced studies after my graduation in July of 2000. And besides, it is to make more research achievement in the life science and at the same time, fulfill my keen dream of standing on the foremost frontline in biology science. Why to choose your University as my graduate school? It is that she is one of the most highly regarded universities in the world and is an ideal place for ambitious youth. Why to select your program? With my experiment skills and broad academic background, I am more and more certain that I am well prepared for the further exploration into your respected program, which is very promising in the modern biology. Despite the highly petition of your admission, I still regard your research program as my future struggling goal. At the time being, I think I am honest, intelligently mature and psychologically ready for this goal and I believe that I can accept the challenge and will surely make success in the end. Would you accept me as one of the members of your program to fulfill my dream? I hope I can contribute my strength and intelligence to your program. You are sure to find that your investment on me is really awarding and worthwhile. Sincerely yours, Yunfei WenDuke Biotechnology statement of purpose生物 20090905 09:28:34 閱讀492 評論0 字號：大中小訂閱 Excited by the idea of being a scientist since I was a small child, my interests inpursuing graduate studies intensified during my undergraduate education and myresearch experience. Rather than discouraging me with the tremendous amount of workand demands for creativity, my college years motivated me to earn a . and tocontinue pursuing a career as a research scientist. Although my exact research goalshave yet to be refined, my interests include studying the genetic basis of disease and thegenetic mechanisms that govern various cellular processes, such as the cell cycle,apoptosis, and tumor formation. Although I entered college without a clear researchdirection, the Biotechnology Program at Rochester Institute of Technology helpeddevelop my interest in the field of Genetics. I found much of my laboratory training atRIT to be extremely rewarding. For example, one experience that early on convinced meto seriously consider genetics was a project involving the construction andcharacterization of a rebinant plasmid. Since I worked in dependently for the mostpart, the project played a key role in increasing my confidence and helping me tounderstand various concepts pertaining to genetic analysis. It was this experience thatmotivated me to seek a position as a teacher抯 assistant for a course in Microbial andViral Genetics. As a TA, I learned how demanding the teaching situation is and howmuch teaching a course can contribute to my own learning. By this time, afterdiscovering my passion for research and the joy of teaching, I began to seriouslycontemplate graduate schools. To excel in graduate school, I decided I would not limitmy experience to my formal education at RIT. Instead, I wanted the opportunity toexplore my career options to make a wellinformed decision as to the area of my futureresearch. With these goals in mind, I chose to take advantage of RIT 抯 CooperativeEducation Program, which