lc-ms液質聯(lián)用資料系列(1)-資料下載頁
2024-12-07 22:04本頁面
【正文】 gram DC/RF (through the apices) ??High Unit Low V (RF) MS Operation Scan Modes Q1 MS Scans Start/Stop Mode Q3 SingleStage MS Scans Start/Stop Mode MS/MS Scan Modes Available Scan Modes – Product Ion Provides structural information – Precursor Ion Provides structural information plementary to Product Ion – Neutral Loss Provides pound class specificity – MRM Used for Quantitation In all MS/MS modes, Q3 is a mass filter – Q3 may scan or be fixed at a given m/z – Q2 is ―source‖ of product ions entering Q3 MS/MS – Product Ion Scan m1+ fixed m3+ scanned MS/MS – Precursor Ion Scan m1+ scanned m3+ fixed MS/MS – Neutral Loss Scan m1+ scanned ???m3+ scanned MS/MS – MRM (Multiple Reaction Monitoring) Precursor ion fixed Fragmentation (CAD) Product ion fixed Fragmentation When an ion fragments the following formulas apply: – Positive mode: – Negative mode: [Precursor]+ [Precursor] ??Product+ ??Product + Neutral + Neutral Two modes of fragmentation on Sciex Instruments: – CAD = Collisionally Activated Dissociation Energy controlled by CE。 occurs in Q2 (collision cell) Ions collide with N2 molecules (CAD gas), collision energy is converted into vibrational energy, and bonds break – CID = CollisionInduced Dissociation Energy controlled by DP。 occurs between orifice and skimmer Ions collide with N2 (from curtain gas) and solvent molecules, collision energy is converted… Ion Detection Detector amp。 Signal Handling Deflector Ion Path Signal Output CEM 1 M? 1000 pF 10 kV Signal Handling Board CEM Horn Voltage () CEM Bias Voltage (+) System Controller (in System Electronics Box)
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