【正文】 is a chlorophyll a/bbinding protein, which plays a key role in plant photosynthesis. A fulllength cDNA encoding Lhca1 gene was cloned from the first strand of Phyllostachys edulis cDNA through RTPCR method, named as LhcaPe01 (GenBank EU035496). 616 bp cDNA fragment was cloned from the first strand of P. iridescens cDNA, named as LhcaH01 (GenBank EU513200) and 613 bp cDNA fragment was cloned from the first strand of P. fimbriligula cDNA, named as LhcaJ01 (GenBank EU513201). The length of LhcaPe01 is 1051bp，which contains an open reading frame encoding 249 amino acids from 39 to 779 positions and has 38bp and 242bp untranslated regions of 5’ end and 3’ end, respectively. There is a 30bp Poly(A) in 3’ end. The P. iridescensPpI and Mw of protein encoded by LhcaPe01 were predicted to be and respectively. The similarity of LhcaPe01 DNA sequence and encoding amino acid sequence blast with barley was the highest in Gramineae family. In Phyllostachys genus, the Lhca1 similarity of P. iridescens, P. fimbriligula and is more higher than that in Gramineae family and the rates is %, % and % respectively. The prediction of the protein structure indicated that Moso bamboo, barley, rice and maize have high similarity.Key words Phyllostachys edulis。結(jié)果表明 LhcaPe01 基因序列從第 39bp 開始到第 779bp 含有 1 個(gè)開放閱讀框（open reading frame, ORF）和一個(gè)中止密碼子，該基因全長(zhǎng) 1051bp，在5’端有 38bp 的非編碼區(qū)（untranslated region, UTR） ，在 3’端含有 242bp 的非編碼區(qū)（untranslated region, UTR）和 Poly(A) 30bp。第一作者：唐文莉，博士生，主要研究方向：竹子生物技術(shù)，電話：010647288776735 Email: 地址：100102 北京朝陽區(qū)望京阜通東大街 8 號(hào) 國(guó)際竹藤網(wǎng)絡(luò)中心。同屬中的角竹（Phyllostachys fimbriligula） ，發(fā)筍能力強(qiáng)，筍味略苦，加工罐頭尤佳，是著名的“晚熟”高產(chǎn)筍用竹，經(jīng)濟(jì)價(jià)值甚高 [12]。引物 3：5’AGTTCGTCGCCATCGCCTTCGCGGAGCA 3’，引物 3 應(yīng)用于 3’末端擴(kuò)增，反應(yīng)條件為：94℃，30s；72℃，3min，5 個(gè)循環(huán)；94℃，30s；70℃,30s ；72℃，3min，5 個(gè)循環(huán)；94℃，30s；68℃,30s ；72℃，3min，25 個(gè)循環(huán)。 Marker 為 200bp DNA Ladder。 Oryza sativa: NM0010640。圖 6中深灰色所示為紅殼竹與毛竹不同的蛋白位點(diǎn)；方框A、B、C、D四個(gè)螺旋區(qū)域是較大保守蛋白折疊區(qū)，其中淺灰色表示潛在的配體葉綠素（potential Chl ligands）和保守的色素結(jié)合位點(diǎn)（conserved pigmentbinding sites） 。在擬南芥中 Lhca Lhca Lhca3 和 Lhca4基因都是以單拷貝形式存在于基因組中，以二聚體形式單獨(dú)地結(jié)合 PSⅠ。2: 818.[10]高素萍,任艷軍,(Phyllostachys)23 個(gè)觀賞竹種間親緣關(guān)系的RAPD分析[J]. 園藝學(xué)報(bào) ,2022,33(3):566570.Gao Suping, Ren Yanjun, Chen Qibing. RAPD Analysis of Geic Relationships among the 23 Ornamental Bamboo Species in Phyllostachys[J]. Acta Horticulturae Sinica。 2022。5,3:15.[8] 高志民,李雪平,彭鎮(zhèn)華,／b結(jié)合蛋白基因全長(zhǎng)的克隆和序列分析[J]. 林業(yè)科學(xué) ,2022, 43(3):3438.Gao Zhimin, Li Xueping, Peng Zhenhua, et al. Cloning and Characterization of a FullLength Gene Encoding the Light Harvesting Chlorophyll a/bBinding Protein in Bamboo[J]. SCIENTIA SILVAE SINICAE。蛋白分子結(jié)構(gòu)特征在研究蛋白功能、進(jìn)行分子設(shè)計(jì)中具有重要作用，近年來隨著基因組學(xué)、蛋白質(zhì)組學(xué)相繼興起，數(shù)以萬計(jì)的蛋白質(zhì)分子三維結(jié)構(gòu)等待解析，但是蛋白質(zhì)分子結(jié)構(gòu)測(cè)定有相當(dāng)難度。本研究中克隆的LhcaPe01 基因，根據(jù)DNAStar軟件預(yù)測(cè)，編碼的蛋白質(zhì)等電點(diǎn)和分子量分別為。 圖4 毛竹LhcaPe01 基因編碼的氨基酸與禾本科其它屬物種的 Lhca1家族基因編碼的氨基酸序列多序列比較 Multiple sequence alignments of animo acids deduced by LhcaPe01 gene of P. edulis and Lhca1 genes of other plants in Gramineae familyThe accession number for Oryza rufipogon :CU406801。質(zhì)粒經(jīng) EcoRⅠ酶切圖譜分析后，進(jìn)行測(cè)序鑒定。引物 1和32 反應(yīng)條件為 94℃，預(yù)變性 5min；94℃，1min；64～70℃，1min；72℃2min，35 個(gè)循環(huán)；72℃延伸 10min。除新疆、內(nèi)蒙、東北、青海等地外，全國(guó)各地均有自然分布或栽培。 Lhca1。1剛竹屬 3 種重要散生竹光系統(tǒng)Ⅰ基因（Lhca1）的克隆、序列分析和蛋白結(jié)構(gòu)預(yù)