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西花薊馬的scar分子檢測(cè)技術(shù)(留存版)

  

【正文】 iiensis、八節(jié)黃薊馬 T. flavidulus、豆大薊馬 Megalurothrips usitatus、茶黃硬薊馬 Scirtothrips dorsalis 和稻簡(jiǎn)管薊馬 Haplothrips aculeatus)田間常見(jiàn)薊馬種類的DNA為模板,進(jìn)行PCR擴(kuò)增。C℃ 45 s;最后 72 176。 9: 茶黃硬薊馬Scirtothrips dorsalis Hood。 2: 1齡幼蟲First 1st instar larva。西花薊馬體型微小, mm左右,~ mm,而且與其他常見(jiàn)的薊馬種類如花薊馬、禾花薊馬、煙薊馬等形態(tài)相似;并且,成蟲常將卵產(chǎn)在寄主植物組織中,而預(yù)蛹期和蛹期主要在土壤中度過(guò)。參考文獻(xiàn)(References)Bailey SF, 1940. The distribution of injurious thrips in the United States. Journal of Economic Entomology, 33: 133136.Bezzara IC, De Resende RO, Pozzer L, Nagata T, Kormelink R, De Avila AC, 1999. Increase of tospoviral diversity in Brazil with the identification of two new tospovirus species, one from chrysanthemum and one from zucchini. Phytopathology, 89(9): 823830.Brunner PC, Fleming C, Frey JE, 2002. A molecular identification key for economically important thrips species (Thysanoptera: Thripidae) using direct sequencing and a PCRRFLPbased approach. Agricultural and Forest Entomology, 4(2): 127136.Callejas C, Velasco A, Gobbi A, Beitia F, Ochando MD, 2005. Fast discrimination (RAPDPCR) of the species forming the pest plex Aleurodicus dispersus–Lecanoideus floccissimus (Hom: Aleyrodidae). Journal of Applied Entomology, 129(7): 382385.Cheng JF, Wan FH, Guo JY, 2006. Analysis of potential distribution of the invaded insect Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) in China. Acta Entomologica Sinica, 49(3): 438446. [程俊峰, 萬(wàn)方浩, 郭建英, 2006. 入侵昆蟲西花薊馬的潛在適生區(qū)分析. 昆蟲學(xué)報(bào), 49(3): 438446]Cockfield SD, Beers EH, Zack RS, 2007. Phenology of western flower thrips Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) on plant species in and near apple orchards in Washington State. Journal of the Entomological Society of British Columbia, 104(11): 3544.De Angelis JD, Sether DM, Rossignol PA, 1993. Survival, development and reproduction in western flower thrips (Thysanptera: Thripidae) exposed to impatiens necrotic spot virus. Environmental Entomology, 22(6): 1 3081 312.De Barro PJ, Driver F, 1997. Use of RAPD PCR to distinguish the B biotype from other biotypes of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). Australian Journal of Entomology, 36: 149152.Gardener MC, Tompkins CM, Whipple OC, 1935. Spotted wilt of truck crops and ornamental plants. Phytopathology, 25: 17.Han YF, 1997. Economic Insect Fauna of China, FASC Vol. 55,. Thysanoptera. Science Press, Beijing. 513 pp, 220 figures. [韓運(yùn)發(fā), 1997. 中國(guó)經(jīng)濟(jì)昆蟲志, 第五十五冊(cè), 纓翅目. 北京: 科學(xué)出版社. 513頁(yè), 220幅圖]Jiang XL, Bai S, Xiao S, Yang B, 2001. Service for the international flower festival in Kunming, China. Plant Quarantine, 15(2): 115117. [蔣小龍, 白松, 肖樞, 楊碧, 2001. 為中國(guó)昆明國(guó)際花卉節(jié)把關(guān)服務(wù). 植物檢疫, 15(2): 115117]Jones DR, 2005. Plant viruses transmitted by thrips. European Journal of Plant Pathology, 113: 119157.Kirk WDJ, Terry LI, 2003. The spread of the western flower thrips Frankliniella occidentalis (Pergande). Agricultural and Forest Entomology, 5: 301310.Li Y, Jia JZ, Wang TY, 1999. Types of molecular markers and their development. Biotechnology Information, (4): 1922. [黎裕, 賈繼增, 王天宇, 1999. 分子標(biāo)記的種類及其發(fā)展. 生物技術(shù)通報(bào), (4): 1922]Moritz G, 2002. The biology of thrips is not the biology of their adults: a developmental view. In: Marullo R, Mound LA eds. Thrips and Tospoviruses. Proceedings of the 7th International Symposium on Thysanoptera, Regio Calabria Italy. 259267.Moritz G, Delker C, Paulsen M, Mound LA, Burgermeister W, 2000. Modern methods for identification of Thysanoptera. EPPO Bulletin, 30: 591593.Moritz G, Mound LA, Morris DC, Goldarazena A, 2004. Pest Thrips of the World: an Identification and Information System Using Molecular and Microscopical Methods. CDROM. Brisbane: Center for Biological Information Technology, University of Queensland. Brisbane, Australia.Patra B, Acharya L, Mukherjee AK, Panda MK, Panda PC, 2008. Molecular characterization of ten cultivars of Canna lilies (Canna Linn.) using PCR based molecular markers (RAPDs and ISSRs). International Journal of Integrative Biology, 2(2): 129137.Robb LK, Parrella PM, 1989. Western flower thrips, a serious pest of floricultural crops. In: Parker LB, Skinner M, Lewis T eds. Towards Understanding Thysanoptera. Proceedings of International Conference on Thrips. February 2123, 1989. Burlington, Vermont. 343358.Toda S, Komazaki S, 2002. Identification of thrips species (Thysanoptera: Thripidae) on Japanese fruit trees by polymerase chain reaction and restriction fragment length polymorphism of the ribosomal ITS2 region. Bulletin of Entomological Research, 92: 359363.Wijkamp I, Almarza N, Goldbach R, Peters D, 1995. Distinct levels of specificity in thrips transmission of tospoviruses.
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