【正文】 on the status of semen analysis in 118 laboratories in China [J]. Asian J Androl, 2010, 12(1): 104110.[6] World Health Organization. WHO Laboratory Manual for the Examination and Processing of Human Semen [M].5th ed. Geneva: World Health Organization, 2010.[7] Pacey AA. Quality assurance and quality control in the laboratory andrology [J]. Asian J Androl, 2010, 12(1): 2125.[8] Baker HW. Status of semen analysis－ment on “A survey on the status of semen analysis in 118 laboratories in China” by JinChun Lu et al. in Asian Journal of Andrology [J]. Asian J Androl, 2010, 12(1): 115117.[9] World Health Organization. WHO Laboratory Manual for the Examination of Human Semen and SpermCervical Mucus Interaction [M]. 4th ed. Cambridge University Press, 1999. 128.[10] Kalz DF, Morales P, Samuels SJ, et al. Mechanisms of filtration of morphologically abnormal human sperm by cervical mucus [J]. Fertil Steril, 1990, 54(3): 513516.[11] Cooper TG, Yeung CH. Computeraided evaluation of assessment of “grade a” spermatozoa by experienced technicians [J]. Fertil Steril, 2006, 85(1): 220224.[12] Auger J. Assessing human sperm morphology: Top models, underdogs or biometrics [J]? Asian J Androl, 2010, 12(1): 3646.[13] Eliasson R. Semen analysis with regard to sperm number, sperm morphology and functional aspects [J]. Asian J Androl, 2010, 12(1): 2632.徐元誠[1] 南京金陵男科醫(yī)院周作民[2] 南京醫(yī)科大學(xué)生殖醫(yī)學(xué)國家級重點(diǎn)實(shí)驗(yàn)室孔令柱[3] 江蘇精誠醫(yī)藥有限公司技術(shù)研發(fā)部徐建平[4] 《臨床檢驗(yàn)雜志》編輯部陸金春[5] 江蘇武警南京醫(yī)院檢驗(yàn)科崔毓桂[6] 江蘇省人民醫(yī)院生殖中心生殖遺傳室9 / 9。精子形態(tài)參數(shù)包括頭部和中段尺寸、頭部橢圓率、勻稱性、頂體面積等。新型CASA很好地解決了這些問題。用鼠標(biāo)點(diǎn)擊任意一條精子，將全屏顯示其運(yùn)動軌跡和9個運(yùn)動參數(shù)。面對某些論文中一系列CASA的數(shù)據(jù)，經(jīng)常會質(zhì)疑其可靠性：該實(shí)驗(yàn)室是否堅(jiān)持了重復(fù)取樣的質(zhì)控原則？檢測結(jié)果是否進(jìn)行了可信區(qū)間的統(tǒng)計(jì)學(xué)處理？實(shí)驗(yàn)操作程序有沒有完整的原始記錄方便查證？如何使國產(chǎn)CASA具有質(zhì)量保證和質(zhì)量控制，這是提高我國精液分析水平和質(zhì)量的關(guān)鍵。（2）實(shí)驗(yàn)步驟繁瑣，花費(fèi)的時間和精力較多，因此可操作性較差。該手冊肯定了精子分析儀（CASA）較人工方法的優(yōu)勢。影像分析具有使精子形態(tài)學(xué)評估量化、客觀性和可重復(fù)性獲得重要進(jìn)展的潛力[6]。手冊第5版（中文版）