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ing in front of me. He just simply said ,”well, I caught sight of this flower and thought that it’s beautiful and you may like it”. I wonder why I was moved because of these simple words and present. Maybe it is the sincerity and the true self that impress me. I thanked him and some colleagues walked past my desk would appreciate the beautiful flower and they noticed that something romance happened there. Then later on, there were some little surprises once in a while. Once, there was something wrong with my drawer and I had plained about it unconsciously. And he fixed it for me. Then the other day, he placed several pink diamond roses beside the flower with a piece of paper saying” I love you more than I can say! Diamond stands for marriage, rose stand for love. I hope you are mine, bee my wife. You are the only girl who I really want to spend the rest of my life to stand by.” Although there were some grammar errors but I got what he meant. He meant so clear and so direct. ll參考文獻(xiàn):[1] 中國科學(xué)院西北植物研究所. 秦嶺植物志[M] 第一卷. 第五冊(cè). 北京: 科學(xué)出版社, 1985.[2] 國家中醫(yī)藥管理局《中華本草》編委會(huì). 中華本草: 第六冊(cè)[M] . 上海科學(xué)技術(shù)出版社, 1999.[3] HOSHIKAY, MURAYAMAN. Analys is of odorants discharged from paederia chinsis[J] . Kogai to Taisaku, 1983, 19(5) : 491 493.[4] 馬養(yǎng)民. 雞矢藤揮發(fā)油化學(xué)成分研究[J]. 西北植物學(xué)報(bào), 2000, 20(1) : 145148.[5] 馬養(yǎng)民. 雞屎藤揮發(fā)油化學(xué)成分和元素的定性鑒定[J] . 陜西林業(yè)科技, 1999, (2) : 1618.[6] 余愛農(nóng), 龔發(fā)俊, 劉定書. 雞屎藤鮮品揮發(fā)油化學(xué)成分的研究[J] . 湖北名族學(xué)院學(xué)報(bào)(自然科學(xué)版) , 2003, 21(1) : 4143.[7] 尹桂豪, 王明月, 曾會(huì)才. 雞屎藤葉中揮發(fā)油的超臨界萃取及氣相色譜質(zhì)譜分析[J] . 食品科技, 2009, 34(12) : 303307.[8] 林雪容, 殷希, 王艷艷, 紀(jì)明慧. 微波輔助提取雞屎藤莖揮發(fā)油及其成分分析[J] . 安徽農(nóng)業(yè)科學(xué), 2011, 39(32) : 19738-19739.[9] 何開家, 劉布鳴, 董曉敏, 陳露, 陳明生, 莫建光. 廣西雞屎藤揮發(fā)油化學(xué)成分GCMSDS 分析研究[J] . 廣西科學(xué), 2010, 17(2) : 138~140, 143. [10]李杰, 劉新平, 徐麗, 宋文建. 超臨界萃取雞矢藤的有效成分[J] . 化工進(jìn)展, 2011, (30) : 288290.[11] 王冬梅, 劉艾琳, 黃林芳, 陳士林. 雞屎藤抑制丁酰膽堿酯酶活性及活性部位的UPLCQTOFMS 分析[J] . 中國醫(yī)科大學(xué)學(xué)報(bào), 2013, 44(1) : 44-48. [12] 吳 強(qiáng), 崔恒敏, 李英倫, 郁建生. 雞屎藤作墊料預(yù)防雛雞白痢的苗醫(yī)理論分析[J] . 黑龍江畜牧獸醫(yī)(科技版) , 2011, (2) : 101 102.[13] 余勉. 雞矢藤化學(xué)成分及藥理作用研究進(jìn)展[J] . 衛(wèi)生職業(yè)教育, 2009, 27 (20) : 149 150.[14] 殷希, 紀(jì)明慧, 吳放心, 田岳申, 夏智高, 萬智恒, 單苗苗. 雞屎藤抗氧化活性成分的提取及其對(duì)羥基自由基的抑制率[J] . 理化檢驗(yàn)化學(xué)分冊(cè), 2010, 48(12) : 14661469.[15] 張加雄, 胡軼娟, 朱軍. HPLC法同時(shí)測(cè)定雞矢藤中熊果酸和齊墩果酸的含量[J] . 現(xiàn)代生物醫(yī)學(xué)進(jìn)展, 2011, 11(24) : 49654967.[16] 張書霞. 雞屎藤的營養(yǎng)成分分析[J] . 食品研究與開發(fā), 2006, 27(3) 150-151.致 謝四年的讀書生活在這個(gè)季節(jié)即將劃上一個(gè)句號(hào),而于我的人生卻只是一個(gè)逗號(hào),我將面對(duì)又一次征程的開始。從上述可分析知甜藤揮發(fā)油的化學(xué)成分存在差異,可能是同種方法在提取過程中,由于條件的設(shè)定不同,造成的差異,還可能是同種方法不同地域差異導(dǎo)致這一成分含量的差異性。2 實(shí)驗(yàn)材料與方法 材料、儀器和試劑 材料甜藤鮮品,采集于凱里 儀器GCMS QP2010SE氣相色譜質(zhì)譜聯(lián)用儀(日本島津),F(xiàn)A2004電子分析天平(上海良平儀表有限公司),MH500調(diào)溫型電熱套(北京科偉永興儀器有限公司),常壓微波合成儀,揮發(fā)油提取儀器 ,冷凝管,圓底燒瓶(500 mL),燒杯(50 mL,500 mL),量筒(10 mL,100 mL),注射器(5 mL),樣品瓶(2個(gè)),膠頭滴管,水管,分液漏斗 試劑乙醚(AR),蒸餾水,無水硫酸鈉 實(shí)驗(yàn)方法 色譜和質(zhì)譜條件氣相色譜條件:RTX5MS彈性石英毛細(xì)管柱(30 m mm μm );柱溫:50 ℃保持1 min,以10 ℃/min升至100 ℃,保持5 min,以10 ℃/min升至240 ℃,保持1 min;汽化室溫度:250 ℃;溶劑延遲:3 min;進(jìn)樣量:1 μL;載氣: He;總流量:5 mL/min;載氣流量:3 mL/min;分流比:1:1質(zhì)譜條件:EI離子源;離子源溫度:200 ℃ ;電子能量:70 eV;檢測(cè)電壓: kV;掃描范圍:29~ 800 amu 甜藤揮發(fā)油的提取方法 水蒸氣法 g,用鑷子將切細(xì)的甜藤莖放入500 mL的圓底燒瓶中,并加入300 mL的蒸餾水,利用揮發(fā)油提取裝置在調(diào)溫型電熱套中加熱,先將電加熱套電壓調(diào)至220 V,沸騰后調(diào)至110 V蒸餾3 h;用100 mL量筒接收揮發(fā)油提取器的成分,然后往圓底燒瓶中加入10 mL 蒸餾水,安裝好裝置并等揮發(fā)油提取器的水積滿再接收,這樣循環(huán)接收,直至得到300 mL的揮發(fā)油提取液即停止加熱,將提取液冷卻,用300 mL乙醚分三次萃取提取液100 mL一次(先將100 mL乙醚置于500 mL的燒杯中,量取的100 mL提取液置于分液漏斗中,將提取液一滴一滴的滴入乙醚中,然后將萃取后的液體進(jìn)行振蕩,靜置后用分液漏斗進(jìn)行分層,棄去下層液體,從漏斗上層倒出乙醚層溶液于500 mL的燒杯中),并往燒杯中加入少量無水硫酸鈉吸水,輕振蕩,靜置,取出有幾層,用旋轉(zhuǎn)蒸發(fā)儀將乙醚層溶液濃縮至2 mL,然后用注射器(5 mL)吸取注入樣品瓶中(編號(hào)為水蒸氣法甜藤莖揮發(fā)油),最后將樣品瓶密封放于室溫保存,用氣象色譜質(zhì)譜聯(lián)用儀進(jìn)行鑒定。馬養(yǎng)民[4]等人通過對(duì)甜藤莖、葉化學(xué)成分的系統(tǒng)預(yù)試發(fā)現(xiàn)其含有揮發(fā)油,%。 Steam Distillation method。 Microwave。根據(jù)植物化學(xué)提取原理,甜藤蒸餾法提取出甜藤的揮發(fā)油,并用化學(xué)分析法對(duì)其所含成分進(jìn)行了定性鑒定,得出甜藤揮發(fā)油中含有酸類、酚類、醛或酮類、酯或內(nèi)酯類以及被還原性成分,包括酯、烴、酮、醛、羧酸和含硫有機(jī)化合物等[5]。 微波輔助法 g,用鑷子將切細(xì)的甜藤莖放入500 mL的圓底燒瓶中,并加入300 mL的蒸餾水,利用揮發(fā)油提取裝置在微波中加熱(條件為溫度:90 ℃、功率:300 J、時(shí)間:90 min),運(yùn)行儀器后將其回流3 h,用100 mL量筒接收揮發(fā)油提取器的成分,然后往提取器中加入10 mL蒸餾水,待提取器中的水循環(huán)一次并進(jìn)行接收(事先計(jì)算10 mL的水按微波加熱的滴速要多久循環(huán)一次),這樣循環(huán)接收,直至得到300 mL的揮發(fā)油提取液即停止加熱,將提取液冷卻,用300 mL乙醚分三次萃取提取液100 mL一次(先將100 mL乙醚置于500 mL的燒杯中,量取的100 mL提取液置于分液漏斗中,將提取液一滴一滴的滴入乙醚中,然后將萃取后的液體進(jìn)行振蕩,靜置后用分液漏斗進(jìn)行分層,棄去下層液體,從漏斗上層倒出乙醚層溶液于500 mL的燒杯中),并往燒杯中加入少量無水硫酸鈉吸水,輕振蕩,靜置,取出有幾層,用旋轉(zhuǎn)蒸發(fā)儀將乙醚層溶液濃縮至2 mL,然后用注射器(5 mL)吸取注入樣品瓶中(編號(hào)為微波輔助法甜藤莖揮發(fā)油),最后將樣品瓶密封放于室溫保存,用氣相色譜質(zhì)譜聯(lián)用儀進(jìn)行鑒定。日本科學(xué)家通過氣相色譜對(duì)甜藤釋放出的氣體成分進(jìn)行了分析,其主要成分有COS、CS2和SO2, 主要含硫有機(jī)成分有CH3SCH3 和CH3SSCH3[3] 。四年的求學(xué)生涯在師長、親友的大力支持下,走得辛苦卻也收獲滿囊,在論文即將付梓之際,思緒萬千,心情久久不能平靜。wait in the line for one hour to get a ticket, and another two hours at the site, to only see a tiny bit of the place due to the crowds. Last year, 428 million tourists traveled in China over the weeklong holiday in October. Traveling during this period is a matter that needs thorough preparation. If you are short on time to plan the uping Golden Week it may not be a bad idea to avoid some of the most crowded places for now. There is always a place so fascinating that everyone yearns for. Arxan is a place like this. The beauty of Arxan is everlasting regardless of the changing of four seasons. Bestowed by nature, its spectacular seasonal landscape and mountains are just beyond word. Arxan is a crucial destination for the remended travelling route, China Inner Mongolia Arxan — Hailar — Manzhouli. It is also the joint of the four prairies across the SinoMongolian border, where people gravitate towards the exotic atmosphere mixed with Chinese, Russian, and Mongolia elements. As a historic site for the Yitian Battle, Arxan still embodies the spirit of Genghis Khan. Walking into Arxan, you will be amazed by a kaleidoscope of gorgeous colors all the year round the Spring azaleas blo