【正文】
l. Without this cosolvent 53% 15bProg was formed。細胞色素 P450酶系 孫 婉菊 2022年 12月 17日 P450酶系概述 ? 細胞色素 P450單加氧酶系 (cytochrome P450 monooxygenases, P450s) : NAD(P)H細胞色素 P450還原酶 (CPR) 細胞色素 P450單加氧酶 (CYP) 2個組分均通過疏水的膜結(jié)合錨點結(jié)合在微粒體膜上。 Towards Preparative Scale Steroid Hydroxylation with Cytochrome P450 Monooxygenase CYP106A2 from Bacillus megaterium ATCC 13368, enables the oxidation of 3keto4ene steroids mainly at position 15. expressed this enzyme together with the electrontransfer partners bovine adrenodoxin and adrenodoxin reductase in Escherichia coli. enzymecoupled cofactor regeneration system was implemented by expressing alcohol dehydrogenase from Lactobacillus br