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河北省工程技術(shù)研究中心評估申請報告(完整版)

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【正文】 eprod. Dev.2005,71(2):154158SCI3Aberrant gene expression in organs of bovine clones that die within two days after birth..李世杰(1)Biol. Reprod2005,72(2):25865.SCI4The proteasomal inhibitor MG132 increases the efficiency of mouse embryo production after cloning by electrofusion李相運(3)Reproduction2005,130(4),553558SCI5A parative study of efficacy of tibolone and simvastatin on atherosclerosis on ovariectomized cholesterolfed rabbit崔亞莉(1)MATURITAS2005,(50):337343SCI6Clofibrate and perfluorodecanoate both upregulate the expression of the pregnane X receptor but oppositely affect its liganddependent induction on cytochrome P4503A23. 馬玉忠(1)Biochemical Pharmacology 2005,69(9): 136371SCI7Afferent and Efferent Connec tions of the Nucleus Rotundus Demonstrated by WGAHRP in the Chick胡滿(1)Anatomia Histologia Embryologia2005,32(6):335340SCI8Morphological Characteristics of Tectal Neurons of Layer I Projecting to the Nucleus Geniculatus Lateralis Ventralis in Chick胡滿(1)Journal of Veterinary Medical Science2005,66(8):1015 1016SCI9Changs in Somal Growth and Dendritic Patterns of the Retinal Ganglion Cells in the Chicks and Chick Embryos胡滿(2)Journal of Veterinary Medical Science2005,65(10):11351137SCI10Changs in the Distribution of Labeled Retinal Ganglion Cells after an Implant of DiI into the Optic Nerve in the Chick Embryos胡滿(2)Journal of Veterinary Medical Science2005, Science, 65 (2): 279281SCI11Singlenucleotide polymorphism identification in the caprine myostatin gene李祥龍(1)Journal of Animal Breeding and Genetics. 2006, 123, 141~144SCI12The red head and neck of Boer goats may be controlled by the recessive allele of the MC1R gene李祥龍(2,通訊作者)Anim. Res.2006, 55: 313–322.SCI13SNP Identification and Analysis in Part of Intron 2 of Goat MSTN Gene and Variation within and among Species.李祥龍(1)Journal of Heredity.2006, 97: 285289.SCI14localization of putative stem cells and four cellpopulations with different differentiation degree in mouse mammary anlagen韓建永(1)Hstochem cell biol. 2006,126:3543SCI15High expression level of soluble SARS spike protein mediated by adenovirus in HEK293 cells仲 飛(1)World Journal of Gastroenterology2006,12(9):14521457SCI16Maternally derived rebinant human antihantavirus monoclonal antibodies are tranferred to mouse樊寶良(3)J. Virol2006,80(8):41834186SCI17Differential glycosylation of rHLf expressed in the mammary gland of transgenic mice樊寶良(3)Animal Biotechnology2006,17: 1320SCI18Cloning of pig parotid secretory protein gene upstream promoter and the establishment of a transgenic mouse model expressing bacterial phytase for agricultural phosphorus pollution control樊寶良(2)Journal of Animal Science2006,84: 513519SCI19Passage number affects the pluripotency of mouse embryonic stem cells as judged by tetraploid embryo aggregation.Li xiangyun(1)Cell Tissue Res 2007, 327(3):607614.SCI20Evolution and Differentiation of MSHR Gene in Different SpeciesLi Xianglong(1)Journal of Heredity,2007, 98 (2): 165168SCI21Vitrification solution containing DMSO and EG can induce parthenogenetic activation of in vitro matured ovine oocytes and decrease sperm penetrationTian Shujun(1)Animal Reproduction Science2007,101 (34):365371SCI22Vitrification of Boer Goat Morulae and Early Blastocysts by Straw and OpenPulled Straw MethodTian Shujun(1)Reproduction Domestic Animal2007,42:3438SCI23Overexpression of the bovine FcRn in the mammary gland results in increased IgG levels in both milk and serum of transgenic miceFan Baoliang(6)Immunology2007, 122(3):4018SCI24Construct synthetic gene encoding artificial spider dragline silk protein and its expression in milk of transgenic mice.Fan Baoliang(2)Animal Biotechnology 2007,18(1):112.SCI25哺乳動物體細胞核移植方法研究進展. 李世杰(3)中國獸醫(yī)學(xué)報2005,25(4):441445國內(nèi)核心26影響波爾山羊超排效果因素桑潤滋(1)中國獸醫(yī)學(xué)報2005,25(5)143146國內(nèi)核心27羔羊小腸pH值及消化酶變化規(guī)律研究張英杰(1)畜牧獸醫(yī)學(xué)報2005,36(2):149-152國內(nèi)核心28品系對小鼠胚胎干細胞分離效率的影響李相運(1)動物學(xué)研究2005,26(3):250-254。毛色深淺可能還受其它位點的控制。其中,標(biāo)記OPO124同時與頸部肌肉重、胃重呈顯著相關(guān);OPQ011同時與頸部肌肉重、肝臟重和板皮面積呈顯著相關(guān)或極顯著相關(guān);OPQ063同時與頸部肌肉重、腿臀肌肉重、腰腹肌肉重呈顯著相關(guān)。1000rpm離心處理有利于精液的低溫保存。該項研究是在已完成的“引入波爾山羊良種基因雜交改良本地山羊試驗研究”的基礎(chǔ)上,通過進一步的級進雜交改良試驗,確定級進雜交改良代數(shù),組建肉用山羊育種核心群,為培育適應(yīng)冀東地區(qū)自然生態(tài)條件的肉山羊新品種奠定基礎(chǔ)。五、經(jīng)濟與社會效益在唐縣富華種羊場、永清縣種羊場、獻縣種羊場、正定、沙河市等地進行了推廣和示范、新疆阿勒泰市、天津北大港農(nóng)場養(yǎng)殖戶已經(jīng)應(yīng)用該成果,目前正逐步向全國推廣。由傳統(tǒng)培養(yǎng)法的5天縮短為5個小時。肉羊寄生蟲病的藥物防治篩選出了防治肉羊球蟲病的高效藥物地克珠利。%,感染率和性別,年齡、不同的生理周期有關(guān)。(二)肉羊疫病的流行規(guī)律研究:綿羊支原體肺炎:%,%。一、立項背景隨著肉羊養(yǎng)殖業(yè)的發(fā)展,肉羊養(yǎng)殖由放牧轉(zhuǎn)變?yōu)樯犸?、半舍?發(fā)病呈上升趨勢,死亡率高,嚴重威脅肉羊養(yǎng)殖業(yè)的健康發(fā)展。七、推廣應(yīng)用的范圍、條件和前景以及存在的問題和改進意見本成果不僅適用奶牛,同樣適用肉牛、水牛。首次系統(tǒng)地研究了供體牛和受體黃牛選擇標(biāo)準(zhǔn),%%,%%。任務(wù)書要求奶牛胚胎移植,鮮胚妊娠率55%以上。超數(shù)排卵:在母畜發(fā)情周期的適當(dāng)時期,對母畜外源注射促性腺激素,使其排出比正常母畜更多的卵子技術(shù)。通過本項技術(shù)的推廣提高了奶牛產(chǎn)奶量和飼料利用率,使奶牛養(yǎng)殖經(jīng)濟效益顯著提高,農(nóng)民收入增加。、低成本犢牛代乳粉新配方。使乳脂中亞油酸含量提高32%~99%,亞麻酸提高166%~245%,共軛亞油酸提高42%~80%。 代表性成果名稱完成單位排序本中心固定人員參加人數(shù)奶牛規(guī)模化飼養(yǎng)關(guān)鍵技術(shù)研究與集成及產(chǎn)業(yè)化示范河北農(nóng)業(yè)大學(xué)(1)李建國(1),李運起(2)代表性成果的主要內(nèi)容、主要的技術(shù)創(chuàng)新和產(chǎn)業(yè)化貢獻。 4.采用中藥現(xiàn)代研究方法,研制出安全高效的肉羊中藥育肥增重劑,增重效果提高65.49%,飼料利用率提高32.74%。該成果為正確指導(dǎo)肉羊生產(chǎn)實踐,提高肉羊業(yè)經(jīng)濟效益,實現(xiàn)肉羊業(yè)的可持續(xù)發(fā)展提供科學(xué)的理論依據(jù)和實用技術(shù)。(4)與企業(yè)聯(lián)合及成果轉(zhuǎn)化能力將科技開發(fā)作為“中心”發(fā)展的經(jīng)濟支柱。發(fā)展目標(biāo)(1)技術(shù)創(chuàng)新、研究與開發(fā)能力“中心”目前具有較強的研究、創(chuàng)新能力,實驗條件比較完備,對該領(lǐng)域國內(nèi)外研究現(xiàn)狀和發(fā)展動態(tài)了解比較清楚,并有較多科研積累,因此,今后“中心”將進一步努力在胚胎工程、分子生物技術(shù)、配子與繁殖調(diào)控領(lǐng)域繼續(xù)保持較強研究、創(chuàng)新能力,計劃每兩年取得一項國內(nèi)先進水平以上的科研成果;力爭在牛、羊體外胚胎生產(chǎn),牛、羊克隆,小鼠胚胎干細胞的分離、克隆等方面有所突破;完成2~3個功能基因的克隆及轉(zhuǎn)基因動物研究;采用MOET技術(shù)育成一個肉羊新品種。附件2:河北省工程技術(shù)研究中心評估申請報告工程中心名稱:河北省牛羊胚胎工程技術(shù)研究中心工程中心主任:桑潤滋 電話:03127521010通訊地址:河北省保定市河北農(nóng)業(yè)大學(xué)動物科技學(xué)院郵政編碼:071001 傳真:03127521010電子郵件地址:rzsang聯(lián)系人:桑潤滋 電話:03127528449依托單位(蓋章):河北農(nóng)業(yè)大學(xué)主管部門(蓋章):河北省教育廳2008年 3 月 26 日填報 簡 表工程中心名 稱河北省牛羊胚胎工程技術(shù)研究中心驗收日期運行補助費(萬元)省科技廳 45 主管部門 45 依托單位 90工程中心主 任姓 名桑
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