離子交換分離原理及設(shè)備-文庫吧在線文庫
【正文】 size ? Resin has fixed size pores which allow molecules smaller than a given size to enter? Small molecules will take longer to pass down the column than large ones because they travel further? Can estimate molecular weight by parison with a standardIon Exchange charge a)Mixture of proteins is applied to the negatively charged column resin – balanced by Na+ ions (red)b)ve and neutral proteins pass in the void volume, +ve proteins stickc)Excess of Na+ ions is addedd)+ve proteins are elutedCould have used change in pH to elute the proteinsCATION EXCHANGEAffinity Chromatography? Uses specific binding property to attach protein to column? Increase concentration of ligand to elute the protein? Examples:– antibody columns monoclonal antibody attached to column– glutathione column – binds glutathioneStransferase on target工業(yè)規(guī)模的色譜分離流程色譜分離設(shè)備玻璃色譜分離柱色譜分離設(shè)備帶熱夾套分離柱 反轉(zhuǎn)式分離柱196。 吸附分離原理及設(shè)
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