【正文】 l crops. In: Parker LB, Skinner M, Lewis T eds. Towards Understanding Thysanoptera. Proceedings of International Conference on Thrips. February 2123, 1989. Burlington, Vermont. 343358.Toda S, Komazaki S, 2002. Identification of thrips species (Thysanoptera: Thripidae) on Japanese fruit trees by polymerase chain reaction and restriction fragment length polymorphism of the ribosomal ITS2 region. Bulletin of Entomological Research, 92: 359363.Wijkamp I, Almarza N, Goldbach R, Peters D, 1995. Distinct levels of specificity in thrips transmission of tospoviruses. Phytopathology, 85(10): 1 0691 074.Wijkamp I, Peters D, 1993. Determination of the median latent period of two tospoviruses in Frankliniella occidentalis, using a novel leaf disk assay. Phytopathology, 83(9): 986991.Xu JJ, Wei LL, 2005. Invaded insect pest—Frankliniella occidentalis first reported in the city of Lincang. Plant Quarantine, 19(5): 294295. [徐家菊, 韋麗莉, 2005. 臨滄市新發(fā)現(xiàn)外來有害生物——西花薊馬. 植物檢疫, 19(5): 294295]You ZH, Lu H, Zhang XS, Feng JN, Shi BC, Gong YJ, Huang DW, 2007. Molecular identification of the introduced western flower thrips, Frankliniella occidentalis (Pergande) and other eight mon thrips species (Thysanoptera: Thripidae). Acta Entomologica Sinica, 50(7): 720726. [游中華, 路虹, 張憲省, 馮紀(jì)年, 石寶才, 宮亞軍, 黃大衛(wèi), 2007. 入侵害蟲西花薊馬及其他 8 種常見薊馬的分子鑒定. 昆蟲學(xué)報(bào), 50(7): 720726]Yudin LS, Cho JJ, Mitcheu WC, 1986. Host range of western flower thrips, Frankliniella occidentalis (Thysanoptera: Thripidae), with special reference to Leucaena glauca. Environment Entomology, 15 (6): 1 2921 295.Zhang WQ, Tong XL, Luo XN, Zhuo WX, 1999. Thysanoptera. In: Huang BK ed. Fauna of Insects in Fujian Province of China. Vol. 1. Fujian Science and Technology Publishing House, Fuzhou. 348395. [張維球, 童曉立, 羅肖南, 卓文禧, 1999. 纓翅目Thysanoptera. 見: 黃邦侃 主編. 福建昆蟲志(第一卷). 福州: 福建科學(xué)技術(shù)出版社. 348395]Zhang YJ, Wu QJ, Xu BY, Zhu GR, 2003. Dangerous alien invasive species, western flower thrips make damages in Beijing. Plant Protection, 29(4): 5859. [張友軍, 吳青君, 徐寶云, 朱國仁, 2003. 危險性外來入侵生物——西花薊馬在北京發(fā)生危害. 植物保護(hù), 29(4): 5859]Zheng CY, Liu YH, Zhang NQ, Zhao XL, 2007. Invaded insect pest—Frankliniella occidentalis first reported in Shandong Province. Journal of Qingdao Agricultural University (Natural Science), 24(3): 172174. [鄭長英, 劉云虹, 張乃芹, 趙希麗, 2007. 山東省發(fā)現(xiàn)外來入侵有害生物——西花薊馬. 青島農(nóng)業(yè)大學(xué)學(xué)報(bào)(自然科學(xué)版), 24(3): 172174]Zhou LB, Liu ZS, Li CY, Ding YM, 2007. Method of the polymerase chain reaction for identification of Frankliniella occidentalis. Plant Quarantine, 21(2): 7881. [周力兵, 劉忠善, 李春艷, 丁元明, 2007. PCR 法鑒定西花薊馬. 植物檢疫, 21(2): 7881]Zhou ZX, Wan FH, Zhang GF, Chen B, 2007. A rapid method for extraction of genomic DNA of Bemisia tabaci. Plant Protection, 33(5): 131133. [周志湘, 萬方浩, 張桂芬, 陳斌, 2007. 煙粉虱基因組DNA快速提取方法. 植物保護(hù), 33(5): 131133]（責(zé)任編輯：袁德成）。如Moritz (2000)采用轉(zhuǎn)錄間隔區(qū)(internal transcribed spacer, ITS)限制片段長度多態(tài)性(ITSRFLP)的方法，先行擴(kuò)增出西花薊馬和美棘薊馬 Echinothrips americanus Morgan的ITS1和ITS2片段，然后選用 5 種內(nèi)切酶消化兩種薊馬的ITS片段，以產(chǎn)生不同的帶型；此方法可以成功地將兩種薊馬區(qū)分開，但是無法將西花薊馬與其他種類的薊馬區(qū)分開。西花薊馬體型微小， mm左右，~ mm，而且與其他常見的薊馬種類如花薊馬、禾花薊馬、煙薊馬等形態(tài)相似；并且，成蟲常將卵產(chǎn)在寄主植物組織中，而預(yù)蛹期和蛹期主要在土壤中度過。. 1: 滿天星花瓣Flowers of baby39。 2: 1齡幼蟲First 1st instar larva。 西花薊馬 SCAR引物的種特異性及靈敏性檢驗(yàn)以西花薊馬及田間采集的其他41種薊馬的DNA為模板，以所設(shè)計(jì)的特異性引物FOMF/FOMR進(jìn)行PCR擴(kuò)增，電泳檢測結(jié)果顯示(圖2)，該引物只對西花薊馬具有擴(kuò)增效果，對其他種類的薊馬不具有擴(kuò)增能力，表明該引物為西花薊馬的特異性引物。 9: 茶黃硬薊馬Scirtothrips dorsalis Hood。 1: 西花薊馬F. occidentalis(Pergande)。C℃ 45 s；最后 72 176。擴(kuò)增反應(yīng)在 ABI9700 PCR基因擴(kuò)增儀上運(yùn)行。然后分別采用上述6 條隨機(jī)引物(上海生工生物工程技術(shù)有限公司協(xié)助合成)，以西花薊馬以及其他 9 種(即花薊馬 F. intonsa、禾花薊馬 F. tenuicornis、煙薊馬 T. tabaci、蔥韭薊馬 T. alliorum、黃胸薊馬 T. hawaiiensis、八節(jié)黃薊馬 T. flavidulus、豆大薊馬 Megalurothrips usitatus、茶黃硬薊馬 Scirtothrips dorsalis 和稻簡管薊馬 Haplothrips aculeatus)田間常見薊馬種類的DNA為模板，進(jìn)行PCR擴(kuò)增。C℃ 12 000 r/min 離心 10 min，取上清液，加入 440 μL預(yù)冷無水乙醇，輕柔混勻后于 30 176。該研究結(jié)果為西花薊馬快速檢測體系的構(gòu)建提供了依據(jù)，為有效阻截西花薊馬在我國的進(jìn)一步擴(kuò)散蔓延和危害提供了技術(shù)保障。近年來分子生物學(xué)技術(shù)發(fā)展迅速，利用分子標(biāo)記技術(shù)只需要少量的DNA就可以快速準(zhǔn)確地的鑒定出薊馬種類，該類技術(shù)不僅可以檢測成蟲，對成蟲前的各個蟲態(tài)亦具有同樣的檢測效能，目前已應(yīng)用于西花薊馬的鑒定研究(Moritz et al., 2000。即使在非適生區(qū)，隨著溫室、大棚等設(shè)施農(nóng)業(yè)的快速發(fā)展，西花薊馬的發(fā)生也會成為可能。 3. Agriculture College, Yangtze University, Jingzhou, Hubei 434025, China)Abstract: The western flower thirps, Frankliniella occidentalis (Pergande), is a worldwide pest, causing serious damage to a wide range of host plants. It was first reported in China in 2003, with a trend of further spreading. Most of thrips species are small in size and similar in morphology, which makes it hard to be distinguished quickly. In the present research, a pair of SCAR (sequence characterized amplified regions) primers (FOMF/FOMR) which are specific to western flower thrips was developed by using other nine familiar thrips species as the control. The fragment amplified by the primers FOMF/FOMR was 3