離子交換分離原理及設(shè)備-免費(fèi)閱讀
【正文】 ? 影響吸附過程的因素 吸附分離原理及設(shè)備 ? 吸附操作及設(shè)備 主要設(shè)備有吸附柱或吸附塔 色譜分離原理及設(shè)備 ? 色譜分離原理 ? 色譜分離操作條件 ? 色譜分離設(shè)備 Column Chromatography Gel Filtration size ? Resin has fixed size pores which allow molecules smaller than a given size to enter ? Small molecules will take longer to pass down the column than large ones because they travel further ? Can estimate molecular weight by parison with a standard Ion Exchange charge a) Mixture of proteins is applied to the negatively charged column resin – balanced by Na+ ions (red) b)ve and neutral proteins pass in the void volume, +ve proteins stick c) Excess of Na+ ions is added d)+ve proteins are eluted Could have used change in pH to elute the proteins CATION EXCHANGE Affinity Chromatography ? Uses specific binding property to attach protein to column ? Increase concentration of ligand to elute the protein ? Examples: – antibody columns monoclonal antibody attached to column – glutathione column – binds glutathioneStransferase on target 工業(yè)規(guī)模的色譜分離流
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