電噴霧四級(jí)桿飛行時(shí)間qtof理論-預(yù)覽頁
【正文】 t eEl z m The aim of an electrostatic reflector, also called reflectron is to improve mass resolution. It creates a retarding field that acts as an ion mirror by deflecting the ions and sending them back through the flight tube. The reflector corrects the energy dispersion of ions with the same m/z ratio. Indeed, ions with more kiic energy will perate the reflectron more deeply and will spend more time in the reflectron. Thus they reach the detector at the same time as slower ions of the same m/z. Ions receive kiic energy from electric field. E = 1/2mv2 Resolution of Ions – TOF Assembly m1 = m2, but E1 E2 Orthogonal Detector accelerator Think of the TOF operation as a drag race between vehicles of different sizes, but all having identical engines: ? “Start line” = orthogonal accelerator。 All Rolling off ?Peak tailing ?Loss of peak resolution ?Decreased peak intensity Rolling 5 To smooth chromatographic data, rolling of one to three is acceptable. Rolling values higher than this can cause considerable tailing and loss of peak shape and separation, which could result in more work to extract data during processing. Rolling does not affect the number of data points and thus does not affect the size of the data file. For data reduction, increase the number of summations. Rolling averaged scans together increases the statistical reliability of your data set. Rolling Averages and Chromatography Too much Rolling Notice: Helpful Tips m/z Species Co n tam i nant 102 (M+H)+ triethy l ami ne (T E A) 123 (M+H)+ di methy l ami nopy ridi ne (D MAP ) 130 (M+H)+ di i s opropyl ethy l a mi ne (D IPE A) 144 (M+H)+ Tr i propyl ami ne ( TPA) 153 (M+H)+ 1,8 di az abi c y c l o[ ]undec 7 en e (D BU ) 182 (M+H)+ di TMS diam i nopy ridi ne 225 449 (M+H)+ (2M+H)+ Dic y c l ohex y l urea ( DC U) 239/241 [( Cl)2 Cl]+ triethy l ami ne 242 M+ tetrabutyl ammoni um (C4H9)4N+ 391 413 454 803 (M+H)+ (M+Na)+ (M+Na+CH3C N)+ (2M+Na)+ Pthal i c ac i d es ter /DO P o r D EHP di i s ooc ty l phthalate (plas ti c i s er) HP L C Flo w R ate ( u L / m i n ) Neb u lizer P r ess u r e ( p s i) Dr y Gas Flo w ( l/ m i n ) Dr y in g Ga s T em p . ( C ) 1 1 0 0 . 5 2 4 150 200 200 – 500 2 4 8 – 1 0 2 0 0 300 Typical ESI Source Conditions Typical Currents (V): Capillary 3000 – 4500 End Plate 500 (up to 2023 with buffers) ESI Common Solvents: ESI Common Buffers Methanol Ethanol Acetic Acid Propanol Isopropanol Formic Acid Butanol Water Ammonium Acetate and Formate Acetone Acetonitrile Sodium Acetate Chloroform Formamide Potassium Acetate Tetrahydrofuran Acetic Acid ESI Solvents Requiring Modifier: Benzene Carbon Disulfide Carbon tetrachloride Cyclohexane Hexane Ligroin Dichloromethane Toluene *DO NOT USE TFA. Useful Conversions 1 mM=1000 mM 1 mM=1 pmol/mL 1 mg/mL= 1 mg/mL To convert mg/mL or mg/mL to pmol/mL : pmol/mL =(mg/mL * 106)/protein MW milli = 103 micro = 106 nano = 109 pico = 1012 femto =1015 atto = 1018 靜夜四無鄰，荒居舊業(yè)貧。 20:49:0120:49:0120:49Wednesday, February 1, 2023 1乍見翻疑夢(mèng)，相悲各問年。 2023年 2月 1日星期三 8時(shí) 49分 1秒 20:49:011 February 2023 1做前，能夠環(huán)視四周；做時(shí)，你只能或者最好沿著以腳為起點(diǎn)的射線向前。 :49:0120:49Feb231Feb23 1世間成事，不求其絕對(duì)圓滿，留一份不足，可得無限完美。 2023年 2月 下午 8時(shí) 49分 :49February 1, 2023 1少年十五二十時(shí)，步行奪得胡馬騎。 20:49:0120:49:0120:492/1/2023 8:49:01 PM 1越是沒有本領(lǐng)的就越加自命不凡。 :49:0120:49:01February 1, 2023 1意志堅(jiān)強(qiáng)的人能把世界放在手中像泥塊一樣任意揉捏。 下午 8時(shí) 49分 1秒 下午 8時(shí) 49分 20:49: MOMODA POWERPOINT Lorem ipsum dolor sit, eleifend nulla ac, fringilla purus. Nulla iaculis tempor felis amet, consectetur adipiscing elit. Fusce id urna blanditut cursus. 感謝您的下載觀看 專家告訴
教學(xué)課件相關(guān)推薦
鄂ICP備17016276號(hào)-1