【正文】
代謝物的氧化過(guò)程中 ATP不產(chǎn)生或產(chǎn)生量減少,剩余能量以熱能的形式放出。 HOW TO EXPRESS UCP1 IN E. COLI? ?Signal peptide ?Avoid of aggregation ?Activator: free fatty acid? ?Cofactor: CoQ? 3) MUTATE ATP SYNTHASE ?Uncouple the ATP synthesis and proton transport ?Target sites: β ,δ etc ?β S174L: decrease enzyme activity but remain the coupling to proton tr