《芯片技術(shù)徐翔麗》ppt課件(文件)
【正文】 度、高分辨率檢測(cè)方法，實(shí)現(xiàn)成像與數(shù)據(jù)分析一體化。 改進(jìn)基質(zhì)材料的表面處理技術(shù)以減少蛋白質(zhì)的非特異性結(jié)合。 Ag, antigen。 Quantitative analysis of human serum leptin using a nanoarray protein chip based on singlemolecule sandwich immunoassay Talanta 78 (2022) 608–612 We report a method for the quantitative analysis of human serum leptin, which is a protein hormone associated with obesity, using a nanoarray protein chip based on a singlemolecule sandwich nanoarray patterning of a biotinprobe with a spot diameter of 150 nm on a selfassembled monolayer functionalized by MPTMS on a glass substrate was successfully acplished using atomic force microscopy (AFM)based dippen nanolithography (DPN). Unlabeled leptin protein molecules in human serum were detected based on the sandwich fluorescence immunoassay by total internal reflection fluorescence microscopy (TIRFM). The linear regression equation for leptin in the range of 100 zM–400 aM was determined to be y = x + 80,382 ( R = ). The accuracy and sensitivity of the chip assay were clinically validated by paring the leptin level in adult serum obtained by this method with those measured using the enzymelinked immunosorbent assay (ELISA) performed with the same leptin standards and serum samples. In cont
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